Photogenerated Radical Pair between Flavin and a Tryptophan-Containing Transmembrane-Type Peptide in a Large Unilamellar Vesicle.

Electron-transfer (ET) reactions in biological systems, such as those with magnetic sensors based on flavoproteins and electron transport at biomembrane interfaces, are interesting and important issues that require understanding. As a model system of flavoproteins in biomimetic environments, we report the dynamics of the radical pair generated by photoinduced ET between riboflavin tetrabutylate (RFTB) and tryptophan (Trp) residues in a transmembrane-type polypeptide, both of which are distributed in a large unilamellar vesicle of 1,2-dimyristoyl--glycero-3-phosphocholine. The Trp residues locate near the hydrophilic membrane interface, as confirmed by a dual-fluorescence quenching assay. The fluorescence and transient absorption upon photoexcitation of RFTB indicate that ET from both the singlet and triplet excited states occurs at the hydrophilic interface, whereas the RFTB in the hydrophobic region does not contribute to ET. The ET efficiency and the magnetic field effect (MFE) on the RFTB anion increase significantly above the gel-to-liquid crystal phase transition temperature due to a decrease in microviscosity. The MFE analysis indicates that the radical pair generated from the triplet ET channel exhibits a long lifetime as those in micellar systems due to the strong cage effect of the vesicle.