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Article Abstract
Background: Bovine tuberculosis (TB) is caused by , a well-known cause of zoonotic tuberculosis in cattle and deer, and has been investigated in many physiological and molecular studies. However, detailed genome-level studies of have not been performed in Korea.
Objectives: To survey whole genome-wide single-nucleotide polymorphism (SNP) variants in Korean field isolates and to define groups in Korea by comparing SNP typing with spoligotyping and variable number tandem repeat typing.
Methods: A total of 46 field isolates, isolated from laryngopharyngeal lymph nodes and lungs of Korean cattle, wild boar, and Korean water deer, were used to identify SNPs by performing whole-genome sequencing. SNP sites were confirmed via polymerase chain reaction using 87 primer pairs.
Results: We identified 34 SNP sites with different frequencies across isolates, and performed SNP typing and epidemiological analysis, which divided the 46 field isolates into 16 subtypes.
Conclusions: Through SNP analysis, detailed differences in samples with identical spoligotypes could be detected. SNP analysis is, therefore, a useful epidemiological tracing tool that could enable better management of bovine TB, thus preventing further outbreaks and reducing the impact of this disease.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007439 | PMC |
| http://dx.doi.org/10.4142/jvs.2021.22.e24 | DOI Listing |
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