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In this study, a soluble complex formed between 0.5% (w/v) heated whey protein isolate (HWPI) and 5% (w/v) octenyl succinic anhydride (OSA)-modified starch at pH 4.5 was used to encapsulate β-carotene for improving its solubility and stability. The apparent aqueous solubility of β-carotene was increased markedly (264.05 ± 72.53 μg/mL) after encapsulation in the soluble complex. Transmission electron microscopy and scanning electron microscopy were used to evaluate the effect of the encapsulation of β-carotene on the structure of the soluble complex. Fourier transform infrared spectroscopy showed that the characteristic peaks of β-carotene disappeared in the soluble complex, suggesting that β-carotene may have been encapsulated into the soluble complex via hydrophobic interactions. X-ray diffraction indicated that the β-carotene was in an amorphous form within the soluble complex. An accelerated stability test showed that the soluble complex could effectively improve the chemical stability of β-carotene during long-term storage under low pH conditions.
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http://dx.doi.org/10.1016/j.foodchem.2021.129267 | DOI Listing |
Commun Chem
September 2025
Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX, USA.
Promiscuity, or selectivity on a spectrum, is an encoded feature in biomolecular anion recognition. To unravel the molecular drivers of promiscuous anion recognition, we have employed a comprehensive approach - spanning experiment and theory - with the Staphylococcus carnosus nitrate regulatory element A (ScNreA) as a model. Thermodynamic analysis reveals that ScNreA complexation with native nitrate and nitrite or non-native iodide is an exothermic process.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, PR China. Electronic address:
As the primary storage protein, highland barley gliadin (HBG) exhibits limitations in the processing of highland barley foods, primarily due to its abundant non-polar amino acids. In this study, HBG was utilized to prepare sugar-HBG complexes with pentose (xylose), hexoses (glucose and galactose), and disaccharides (lactose and maltose) in an aqueous system at a pH of 11 and a temperature of 75 °C. Subsequently, the structural and functional characteristics of these complexes were evaluated.
View Article and Find Full Text PDFJ Reprod Immunol
September 2025
Laboratory of Immunology, Department of Biology, University of Crete, University Campus, Heraklion 70013, Crete, Greece. Electronic address:
Except from the myeloid in origin cells, ectopic expression of T-cell receptors (TCRs) was also detected in sperm and the female reproductive tract, which in conjunction with major histocompatibility complex (MHC) molecules suggested their involvement in mate choice. Following-up these observations and considering that MHC/TCR interactions could guide spermatozoa towards ovum, the present study aimed to delineate the presence of TCRs in sperm vis-à-vis cognate recognition and define such expression during spermatogenesis. Immunofluorescence experiments using fertile BALB/c males showed that despite the inbred origin of mice, all combinations of high (HI, >20 % expression) and low (LO, <5 % expression) TCRαβ and TCRγδ expression could be detected in equal distribution rates, followed by an inverse pattern of MHC expression.
View Article and Find Full Text PDFMater Today Bio
October 2025
Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Max Bergmann Center of Biomaterials Dresden, 01069, Dresden, Germany.
Glycosaminoglycan-based biohybrid hydrogels represent a powerful class of cell-instructive materials with proven potential in tissue engineering and regenerative medicine. Their biomedical functionality relies on a nanoscale polymer network that standard microscopy techniques cannot resolve. Here, we introduce an advanced analytical approach that integrates transmission electron microscopy, X-ray scattering, and computer simulations to directly and quantitatively characterize the nanoscale molecular network structure of these hydrogels.
View Article and Find Full Text PDFDalton Trans
September 2025
Department of Chemistry, Istanbul Technical University, Maslak, Istanbul, 34469, Türkiye.
A novel phthalonitrile derivative (a) containing three functional groups (hexyl, aminated ester, phenoxy) was synthesized and subsequently cyclotetramerized in the presence of the corresponding metal chloride salts to obtain hexadeca-substituted metal {M = Cu(II) and Co(II)} phthalocyanines (b and c). The water-soluble phthalocyanines (d and e) were prepared by treating the newly synthesized complexes (b and c) with methyl iodide. Moreover, gold nanoparticles (1) and silver nanoparticles (2) were prepared, and their surfaces were modified with quaternary phthalocyanines (d and e).
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