Mutations in the gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

MicroPubl Biol

Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093-0116, USA.

Published: January 2021


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Article Abstract

To facilitate genetic mapping of developmental mutants of , we produced a genetic marker that combines recessive auxotrophy with dominant positive selection. We first identified the gene affected by the auxotrophic mutation and then replaced it with a cassette that confers antibiotic resistance. This strain may be used to produce bi-parental somatic hybrids with nearly any other strain.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841435PMC
http://dx.doi.org/10.17912/micropub.biology.000364DOI Listing

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