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We summarize different levels of RNA structure prediction, from classical 2D structure to extended secondary structure and motif-based research toward 3D structure prediction of RNA. We outline the importance of classical secondary structure during all those levels of structure prediction.
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http://dx.doi.org/10.1042/ETLS20160027 | DOI Listing |
Proc Natl Acad Sci U S A
September 2025
Martin A. Fisher School of Physics, Brandeis University, Waltham, MA 02453.
Programmable self-assembly has recently enabled the creation of complex structures through precise control of the interparticle interactions and the particle geometries. Targeting ever more structurally complex, dynamic, and functional assemblies necessitates going beyond the design of the structure itself, to the measurement and control of the local flexibility of the intersubunit connections and its impact on the collective mechanics of the entire assembly. In this study, we demonstrate a method to infer the mechanical properties of multisubunit assemblies using cryogenic electron microscopy (cryo-EM) and RELION's multi-body refinement.
View Article and Find Full Text PDFJ Histotechnol
September 2025
3d.FAB, Université Claude Bernard Lyon 1, CNRS, INSA, CPE-Lyon, Villeurbanne, France.
Histological preparation paraffin embedding is the gold standard method for evaluating tissue structure and composition, whether it is originated from biopsy or engineered . Quite often, deformation and shrinkage occur during the histological preparation, which are difficult to predict and qualify. The present study investigates the morphometric changes in bioprinted hydrogels composed of alginate and gelatine, common tissue engineering materials, focusing on three morphologies: full slabs, porous slabs, and porous cubes.
View Article and Find Full Text PDFNanoscale
September 2025
School of Materials Science and Engineering, Beihang University, Beijing 100191, China.
The challenge of photocatalytic hydrogen production has motivated a targeted search for MXenes as a promising class of materials for this transformation because of their high mobility and high light absorption. High-throughput screening has been widely used to discover new materials, but the relatively high cost limits the chemical space for searching MXenes. We developed a deep-learning-enabled high-throughput screening approach that identified 14 stable candidates with suitable band alignment for water splitting from 23 857 MXenes.
View Article and Find Full Text PDFAppl Biochem Biotechnol
September 2025
School of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Recombinant DNA technology is widely used to produce industrially and pharmaceutically important proteins. In silico analysis, performed before executing wet lab experiments has been greatly helpful in this connection. A shift in protein analysis has been observed over the past decade, driven by advancements in bioinformatics databases, tools, software, and web servers.
View Article and Find Full Text PDFInt Urol Nephrol
September 2025
Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Purpose: Living donor kidney transplantation is a critical strategy to address the growing burden of end-stage kidney disease (ESKD) in Malaysia. Whilst living donation is generally safe, concerns remain regarding long-term donor outcomes. This study aimed to evaluate renal function and morbidity changes in living kidney donors 1 year post-donation, and to identify predictors of impaired kidney function.
View Article and Find Full Text PDF