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Objective: To explore the diagnosis, classification and treatment of ectopic seminal tract opening in enlarged prostatic utricle (EPU).
Methods: We retrospectively analyzed the clinical data on 22 cases of ectopic seminal tract opening in EPU confirmed by spermography, EPU open cannula angiography or intraoperative puncture of the vas deferens and treated by transurethral incision of EPU, cold-knife incision or electric incision of EPU, full drainage of the anteriorwal, and open or laparoscopic surgery from October 1985 to October 2017.
Results: Five of the patients were diagnosed with ectopic opening of the vas deferens and the other 17 with ectopic opening of the ejaculatory duct in EPU. During the 3-48 months of postoperative follow-up, symptoms disappeared in all the cases, semen quality was improved in those with infertility, and 2 of the infertile patients achieved pregnancy via ICSI.
Conclusions: Ectopic seminal tract opening in EPU is rare clinically. Spermography is a reliable method for the diagnosis of the disease, and its treatment should be aimed at restoring the smooth flow of semen based on proper classification and typing of the disease.
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J Reprod Immunol
September 2025
Laboratory of Immunology, Department of Biology, University of Crete, University Campus, Heraklion 70013, Crete, Greece. Electronic address:
Except from the myeloid in origin cells, ectopic expression of T-cell receptors (TCRs) was also detected in sperm and the female reproductive tract, which in conjunction with major histocompatibility complex (MHC) molecules suggested their involvement in mate choice. Following-up these observations and considering that MHC/TCR interactions could guide spermatozoa towards ovum, the present study aimed to delineate the presence of TCRs in sperm vis-à-vis cognate recognition and define such expression during spermatogenesis. Immunofluorescence experiments using fertile BALB/c males showed that despite the inbred origin of mice, all combinations of high (HI, >20 % expression) and low (LO, <5 % expression) TCRαβ and TCRγδ expression could be detected in equal distribution rates, followed by an inverse pattern of MHC expression.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.
Ovulation is an intricate process that is essential for reproductive success. In , ovulation increases after mating. This increase is initiated by the male seminal fluid protein ovulin and is executed by female pathways, including octopamine (OA) neuronal signaling.
View Article and Find Full Text PDFMol Biol Evol
September 2025
Department of Evolution and Ecology, University of California, Davis, CA, USA.
Drosophila seminal fluid proteins (SFPs) are often cited as an example of interlocus sexual conflict, wherein the proteins increase male fitness while decreasing female fitness, spurring recurring female counter adaptations and rapid molecular evolution. This model predicts that male-expressed genetic variation in the accessory gland, which produces seminal fluid, should generate counter-evolving genetic pathways in females, resulting in sexual coevolution. Using a trio of D.
View Article and Find Full Text PDFMol Hum Reprod
September 2025
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India.
Human fertilization is a coordinated process involving nteraction of sperm with the oocyte. As the sperm pass through the female reproductive tract (FRT), they are presented with numerous challenges. These include navigating through highly viscous cervical mucus while evading immune responses to successfully fertilize the oocyte.
View Article and Find Full Text PDFbioRxiv
August 2025
Instituto de Investigaciones Biológicas (IIB-FCEyN/CONICET), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mar del Plata, Argentina.
Sperm capacitation involves proteolytic remodeling of membrane proteins, including components of the CatSper calcium channel, which is essential for hyperactivation and male fertility. Here, we identify the seminal protease inhibitor SPINK3, a known decapacitation factor that suppresses premature capacitation in the female tract, as the first physiological inhibitor of CATSPER1 processing. In mouse sperm, SPINK3 blocks capacitation-induced CATSPER1 cleavage, preserving a subpopulation with intact CatSper channels and lacking pTyr development in the flagellum.
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