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Saccharomyces cerevisiae is the main actor of wine fermentation but at present, still little is known about the factors impacting its distribution in the vineyards. In this study, 23 vineyards and 7 cellars were sampled over 2 consecutive years in the Bordeaux and Bergerac regions. The impact of geography and farming system and the relation between grape and vat populations were evaluated using a collection of 1374 S. cerevisiae merlot grape isolates and 289 vat isolates analyzed at 17 microsatellites loci. A very high genetic diversity of S. cerevisiae strains was obtained from grape samples, higher in conventional farming system than in organic one. The geographic appellation and the wine estate significantly impact the S. cerevisiae population structure, whereas the type of farming system has a weak global effect. When comparing cellar and vineyard populations, we evidenced the tight connection between the two compartments, based on the high proportion of grape isolates (25%) related to the commercial starters used in the cellar and on the estimation of bidirectional geneflows between the vineyard and the cellar compartments.
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http://dx.doi.org/10.1038/s41598-020-73279-7 | DOI Listing |
Plant Dis
September 2025
Shenyang Agricultural University, College of Plant Protection, Nematology Institute of Northern China, Shenyang, China;
Root-knot nematodes (Meloidogyne spp.) cause catastrophic yield losses in global agriculture. This study identified itaconic acid (IA), through comparative metabolomic analysis (the study of small molecules in biological systems), as a key virulence-related metabolite produced by the fungus Trichoderma citrinoviride Snef1910.
View Article and Find Full Text PDFLife Sci Alliance
November 2025
Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan
Mass-based fingerprinting can characterize microorganisms; however, expansion of these methods to predict specific gene functions is lacking. Therefore, mass fingerprinting was developed to functionally profile a yeast knockout library. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) fingerprints of 3,238 knockouts were digitized for correlation with gene ontology (GO).
View Article and Find Full Text PDFNucleic Acids Res
September 2025
Expression génétique microbienne, UMR8261 CNRS, Université Paris Cité, Institut de Biologie Physico-Chimique, Paris 75005, France.
Targeted gene editing can be achieved using CRISPR-Cas9-assisted recombineering. However, high-efficiency editing requires careful optimization for each locus to be modified, which can be tedious and time-consuming. In this work, we developed a simple, fast and cheap method: Engineered Assembly of SYnthetic operons for targeted editing (EASY-edit) in Escherichia coli.
View Article and Find Full Text PDFComp Biochem Physiol C Toxicol Pharmacol
September 2025
Çukurova University, Biotechnology Research and Application Center, Adana, Turkey; Çukurova University, Faculty of Fisheries, Department of Aquaculture, 01250, Adana, Turkey. Electronic address:
Pyridaben (PDB) is a widely used acaricide in agriculture, classified as highly toxic to aquatic life (H400, H410; USEPA) because it inhibits mitochondrial complex I. This study aimed to evaluate the subacute toxicity of PDB (0.20-0.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul, 08826, Republic of Korea; Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address:
The intestinal immune microenvironment plays a crucial role in regulating systemic immune responses and is implicated in various diseases. Nevertheless, no existing model simultaneously replicates the three-dimensional (3D) immune microenvironment and the mucosal barrier. This study presents a novel mucosa-mimic model that consists of a cell-laden hydrogel matrix and a pseudo-mucus layer that emulate the intestinal lamina propria and mucosal barrier, respectively.
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