Genes and , Coding for Cytokinin Biosynthesis Enzymes, Are Essential for Tumorigenesis and Growth by pv. savastanoi NCPPB 3335.

The phytopathogenic bacterium pv. savastanoi elicits aerial tumors on olive plants and is also able to synthesize large amounts of auxins and cytokinins. The auxin indoleacetic acid was shown to be required for tumorigenesis, but there is only correlational evidence suggesting a role for cytokinins. The model strain NCPPB 3335 contains two plasmid-borne genes coding for cytokinin biosynthesis enzymes: , for an isopentenyl transferase and , for an isopentenyl-diphosphate delta-isomerase. Phylogenetic analyses showed that carriage of and is not strictly associated with tumorigenic bacteria, that both genes were linked when first acquired by , and that a different allele of has been independently acquired by pv. savastanoi and closely related bacteria. We generated mutant derivatives of NCPPB 3335 cured of virulence plasmids or with site-specific deletions of genes and/or and evaluated their virulence in lignified and micropropagated olive plants. Strains lacking , , or both produced tumors with average volumes up to 29 times smaller and reached populations up to two orders of magnitude lower than those induced by strain NCPPB 3335; these phenotypes reverted by complementation with the cloned genes. -zeatin was the most abundant cytokinin in culture filtrates of NCPPB 3335. Deletion of gene abolished biosynthesis of -zeatin and dihydrozeatin, whereas a reduced but significant amount of isopentenyladenine was still detected in the medium, suggesting the existence of other genes contributing to cytokinin biosynthesis in . Conversely, extracts from strains lacking gene contained significantly higher amounts of -zeatin than extracts from the wild-type strain but similar amounts of the other cytokinins. This suggests that Idi might promote tumorigenesis by ensuring the biosynthesis of the most active cytokinin forms, their correct balance , or by regulating the expression of other virulence genes. Therefore, gene , but not gene , is essential for the biosynthesis of high amounts of cytokinins in culture; however, both and are individually essential for the adequate development of tumors on olive plants by Psv NCPPB 3335.