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A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments.
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http://dx.doi.org/10.1016/j.mimet.2020.106053 | DOI Listing |
Appl Biochem Biotechnol
September 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Marine-derived enzymes often show distinct physiological properties and great potential for industrial use. Salt ions may improve the stability and expression efficiency of marine enzymes, which requires salt-resistant host based expression platform. Aspergillus oryzae of good protein expression and secretion was evaluated and explored for this purpose.
View Article and Find Full Text PDFHigh Blood Press Cardiovasc Prev
September 2025
Center for Translational and Experimental Cardiology, Department of Cardiology, University Hospital Zurich and University of Zürich, Wagistrasse 12, 8952, Schlieren, Switzerland.
Introduction: Epigenetic changes are important modulators of gene expression. The histone acetyltransferase gene non-derepressible 5 (Gcn5) is emerging as a pivotal epigenetic player in metabolism and cancer, yet its role in obesity and cardiovascular disease remains elusive.
Aims: To investigate Gcn5 role in obesity-related endothelial dysfunction.
J Anim Sci
September 2025
Department of Animal Biotechnology, Dankook University, Cheonan, 31116, Republic of Korea.
The post-weaning period is stressful for pigs due to changes in their environment and diet. The occurrence of diarrhea at this stage is high. Growth promoters such as antibiotics and zinc oxide (ZnO) have been used to not only reduce post-weaning diarrhea but also improve growth performance of weaning pigs.
View Article and Find Full Text PDFFront Plant Sci
September 2025
College of Life Sciences, Engineering Research Center for High-Valued Utilization of Fruit Resources in Western China of Ministry of Education, Shaanxi Normal University, Xi'an, China.
Plant seeds have evolved diverse dormancy types and regulatory mechanisms to adapt to environmental conditions and seasonal changes. As a commonly used rootstock for cultivated pears, faces challenges in seedling production and large-scale cultivation due to limited understanding of seed dormancy mechanisms. In this study, we report that seeds exhibit non-deep physiological dormancy, with seed coats playing a pivotal regulatory role.
View Article and Find Full Text PDFNat Cell Biol
September 2025
Dioscuri Centre for Chromatin Biology and Epigenomics, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Topologically associating domains (TADs) and chromatin architectural loops impact promoter-enhancer interactions, with CCCTC-binding factor (CTCF) defining TAD borders and loop anchors. TAD boundaries and loops progressively strengthen upon embryonic stem (ES) cell differentiation, underscoring the importance of chromatin topology in ontogeny. However, the mechanisms driving this process remain unclear.
View Article and Find Full Text PDF