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http://dx.doi.org/10.1007/s11427-020-1805-0 | DOI Listing |
Int J Mol Sci
August 2025
Stefan S. Nicolau Institute of Virology, 030304 Bucharest, Romania.
There is an increasing need to understand the long-term dynamics and quality of SARS-CoV-2 immune memory-both humoral and cellular-particularly with emerging variants. This study aimed to evaluate immune durability and variant-specific modulation through a longitudinal analysis of individuals with diverse SARS-CoV-2 exposure histories, over two years after infection and/or vaccination. The study involved assessing anti-spike IgG and IgA levels over time and analyzing their relationship with neutralizing activity against both ancestral and Omicron SARS-CoV-2 variants.
View Article and Find Full Text PDFVaccines (Basel)
July 2025
Unitat de Suport a la Recerca Metropolitana Nord, Institut Universitari d'Investigació en Atenció Primària Jordi Gol (IDIAP Jordi Gol), Mare de Déu de Guadalupe 2, Planta 1ª, Mataró, 08303 Barcelona, Spain.
: SARS-CoV-2 booster vaccination remains essential to prevent severe COVID-19, particularly in vulnerable populations such as older adults. This study evaluated the durability and dynamics of immune responses following booster vaccination(s) in >65-year-old individuals and examined their association with protection against new infections. : Immune responses were evaluated at 3, 9, and 15 months post-booster, measuring SARS-CoV-2-specific IgG antibodies against spike [IgG(S)] and nucleocapsid [IgG(N)] proteins, neutralizing activity against the Omicron BA.
View Article and Find Full Text PDFMicrobiol Spectr
August 2025
Department of Virology, Medical Laboratory, Dr. Gernot Walder GmbH, Ausservillgraten, Austria.
Unlabelled: Total RNA-seq allows for the comprehensive detection of differentially expressed genes (DEGs), providing insight into immune responses during infection. In this study, we addressed the question of whether total RNA-seq can detect differences in gene expression and, therefore, immune responses between infection-naïve and imprinted patients. By sequencing all DEGs in the acute phase of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron B.
View Article and Find Full Text PDFMicrobiol Spectr
July 2025
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
At the COVID-19 pandemic onset, convalescent plasma, collected from patients recovered from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, emerged as a potential treatment option, but the effect on the patient immune response was unclear. To determine treatment effect on the infection antibody response, plasma samples were collected through 90 days from 104 SARS-CoV-2-infected participants after receiving CCP or control plasma, as part of a multicenter, double-blind, randomized, controlled early treatment trial. Enzyme immunoassay (EIA) was used to quantify binding IgG, IgA, and IgM to nucleocapsid (N) protein and IgG to the USA-WA1/2020 spike (S) protein.
View Article and Find Full Text PDFJ Steroid Biochem Mol Biol
October 2025
Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB CONICET-UNVM). Campus Universitario, Av. Arturo Jauretche 1555. Villa María, Córdoba 5900, Argentina; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas, Universidad Nacional Vil
Vaccination was critical in controlling the transmission of SARS-CoV-2 during the COVID-19 pandemic. Nevertheless, immune responses are also influenced by nutritional and endocrine factors, particularly vitamin D, whose deficiency has been linked to an increased risk and severity of respiratory infections. This study aimed to assess the impact of 25-hydroxyvitamin D (25(OH)D) levels on the humoral immune response to SARS-CoV-2, as measured by specific immunoglobulin G (IgG) antibody levels.
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