Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Reactive oxygen species (ROS) could have a negative impact on sperm cellular function and viability. This chapter describes a protocol for oxidative stress evaluation using dichlorofluorescein (DCF) which can specifically reveal intracellular reactive oxygen species. The protocol described here has been used in human and teleost species sperm samples. The method can be used with two approaches: (1) flow cytometry, for quantification of DCF cells, or (2) confocal microscopy, for the localization of ROS within the cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-0896-8_8 | DOI Listing |
Publication Analysis
Top Keywords
flow cytometry
8
confocal microscopy
8
reactive oxygen
8
oxygen species
8
cytometry confocal
4
microscopy ros
4
ros evaluation
4
evaluation fish
4
fish human
4
human spermatozoa
4