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Considering angle diversity and synthetic aperture, Fourier ptychographic microscopy (FPM) could address contradiction of high resolution and wide field of view. However, in the conventional FPM method, large capture quantity leads to poor efficiency. So, an efficient FPM method based on optimized pattern of LED angle illumination is proposed. Firstly, from position relationship between the LED, aperture and sample, we obtain the theoretical expandable spectrum range and the spectrum distribution in Fourier space of all LEDs. Secondly, we u se image quality assessment methods to extract differential expressions between an arbitrary LED illumination and full LEDs illumination. Thirdly, the optimized angle illumination strategy is achieved according to the analysis of different expressions. Based on this method, we design a rhombus-based illumination method for the LED array to accelerate the FPM efficiency. Finally, we validate the effectiveness and efficiency of our method with both simulated and real experiments. The results indicate that our method can effectively improve the efficiency for FPM without sacrificing image reconstruction quality.
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http://dx.doi.org/10.1016/j.micron.2020.102920 | DOI Listing |
Eur J Pharm Biopharm
September 2025
Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, United Kingdom. Electronic address:
To ensure safety, pharmaceuticals are rigorously tested for lipopolysaccharide (LPS) contamination, as this can trigger severe immune reactions in patients. Low Endotoxin Recovery (LER), describing the masking of spiked LPS controls in Limulus Amebocyte Lysate (LAL) assays, has been associated with the presence of chelating agents and surfactants in pharmaceutical formulations. The addition of excipients, such as Mg2, have shown the ability to mitigate the effects of LER, however, inconsistencies in various studies regarding the influence of the excipients on LPS aggregate characteristics and LER occurrence hinder a clear understanding of the mechanisms underlying LER.
View Article and Find Full Text PDFLangmuir
September 2025
School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, People's Republic of China.
The study of the self-assembly of surfactants in aqueous solutions, though a traditional field, remains fascinating and full of novelty. In this article, the anionic perfluorodecanoic acid surfactant (PFA) is separately complexed with three hydroxyalkylamines (monoethanolamine (MEA), diethylamine (DEA), and triethanolamine (TEA)) in aqueous solutions. The transformation of aggregate morphologies from spherical unilamellar to nanotubes and then to spherical bilamellar is observed at room temperature, which is confirmed by cryo-transmission electron microscopy (cryo-TEM).
View Article and Find Full Text PDFAngew Chem Int Ed Engl
September 2025
Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
Photodegradable nanoparticles with sphere, worm, and vesicle morphologies were synthesized following polymerization induced self-assembly (PISA), incorporating a photoresponsive phenyl vinyl ketone (PVK) block and a nonphoto responsive 2-hydroxypropyl methacrylamide (HPMA) block. The photodegradation of nanoparticles under UV revealed that the initial shapes of sphere and vesicle particles are retained even until 7 h and after 24 h of photo-induced degradation, respectively, despite a significant reduction in molecular weight (M). This could be due to the assembly of degraded PVK fragments in the hydrophobic region, maintaining the relative hydrophilic to hydrophobic ratio.
View Article and Find Full Text PDFVestn Oftalmol
September 2025
OOO Diagnosticheskij tsentr Zreniye, Saint Petersburg, Russia.
Objective: This study evaluated the effect of sequential therapy with different dosages of Mexidol on the stabilization of glaucomatous optic neuropathy (GON) in patients with primary open-angle glaucoma (POAG).
Material And Methods: The study included 80 patients (160 eyes) with stage II and III POAG, randomized into three groups comparable by age, gender, and distribution of glaucoma stage. All patients received sequential therapy with Mexidol (14 days parenterally followed by 90 days orally).
Sleep Adv
July 2025
Lindner Center of HOPE Research Institute, Mason, OH, United States.
Study Objectives: Dim light melatonin onset (DLMO) is the best-established marker of central circadian phase and may contribute to unraveling the role of the circadian system in obesity. This study evaluated DLMO among individuals with obesity using a home-based assessment and explored its clinical correlates and procedural variations.
Method: Fifty-eight women (mean [SD] age 40.