Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This study describes the adaptation of non-linear microscopy for single-particle tracking (SPT), a method commonly used in biology with single-photon fluorescence. Imaging moving objects with non-linear microscopy raises difficulties due to the scanning process of the acquisitions. The interest of the study is based on the balance between all the experimental parameters (objective, resolution, frame rate) which need to be optimized to record long trajectories with the best accuracy and frame rate. To evaluate the performance of the setup for SPT, several basic estimation methods are used and adapted to the new detection process. The covariance-based estimator (CVE) seems to be the best way to evaluate the diffusion coefficient from trajectories using the specific factors of motion blur and localization error.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466504 | PMC |
| http://dx.doi.org/10.3390/nano10081519 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High Efficiency Labeling of nerve Fibers in cleared tissue for light-sheet microscopy.
J Neurosci Methods
September 2025
European Laboratory for Non-linear Spectroscopy, via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; National Institute of Optics -National Research Council (CNR-INO), 50125 Sesto Fiorentino, Italy. Electronic address:
Background: Tissue clearing techniques combined with light-sheet fluorescence microscopy (LSFM) enable high-resolution 3D imaging of biological structures without physical sectioning. While widely used in neuroscience to determine brain architecture and connectomics, their application for spinal cord mapping remains more limited, posing challenges for studying demyelinating diseases like multiple sclerosis. Myelin visualization in cleared tissues is particularly difficult due to the lipid-removal nature of most clearing protocols, and alternative immunolabeling approaches failed to reach satisfying results.
View Article and Find Full Text PDFRobust representation and non-linear spectral integration of simple and complex harmonic sounds in layers 4 and 2/3 of primary auditory cortex.
bioRxiv
August 2025
Department of Biomedical Engineering, Johns Hopkins University School of Medicine.
Sound harmonicity is foundational in complex auditory stimuli like music and vocalizations but it remains unclear how such spectrally complex stimuli are processed in the auditory cortex (ACtx). Subregions of the auditory cortex process are thought to process harmonic stimuli differently, and secondary ACtx (A2) layer (L) 2/3 is believed to be the most selective. Selective responses to sound features in ACtx are thought to emerge hierarchically starting from A1 L4.
View Article and Find Full Text PDFIlluminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction.
Microsc Res Tech
July 2025
Department of Experimental and Clinical Medicine (DMSC), Section of Physiological Sciences, University of Florence, Florence, Italy.
In this letter, we put forward the light sheet fluorescence microscopy (LSFM) as a cutting-edge tool for 3D imaging of whole skeletal muscle, focusing on satellite cells (SCs). SCs represent the resident adult muscle stem cells, normally lying quiescent between the sarcolemma of the myofiber and the surrounding basal lamina. They typically express Pax-7 and, when activated following damage, they sequentially express specific myogenic regulatory factors including the myogenic determination factor, MyoD, thus starting differentiation towards multinucleated myofibers to repair injured tissue.
View Article and Find Full Text PDFFrom microstructure to mechanics: A multi-constituent micro-scale computational model of arterial tissue.
Comput Biol Med
September 2025
Dpt. Mechanical Engineering, KU Leuven, Belgium; FIBEr, KU Leuven Core Facility for Biomechanical Experimentation, Belgium. Electronic address:
Arterial tissues exhibit complex growth and remodeling processes, where cells continuously regulate extracellular matrix (ECM) components in response to mechanical and biochemical stimuli. Understanding the interplay between the arterial microstructure and mechanical behavior is crucial for characterizing cardiovascular diseases, such as hypertension and atherosclerosis. In this study, we developed a microstructure-based representative volume element (RVE) model of the porcine aortic medial layer, incorporating elastic lamellae, elastin fibers, collagen fibers, and smooth muscle cells.
View Article and Find Full Text PDFAdipocyte mitochondria in dairy cows reveals constraints in growth and signals adaptive metabolic responses.
Sci Rep
August 2025
Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania.
Understanding the dynamics of metabolic regulation in the body requires an in-depth examination of the distribution and characteristics of mitochondrial populations within adipose tissue. This study aimed to investigate the correlation between mitochondrial size and the expansion of white adipocytes across different Body Condition Scores (BCS) in dairy cows. BCS was used to categorize cows into three groups: cachectic, normal weight, and overweight.
View Article and Find Full Text PDF