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The study focuses on predictive modelling of inactivation of after treatment with chlorophyllin-chitosan complex and visible light. cells were incubated with chlorophyllin-chitosan complex (0.001% chlorophyllin and 0.1% chitosan) for different times (5-60 min) and then illuminated with visible light (=405 nm, =38 J/cm). Inactivation curves and post-treatment regrowth curves were built based on microbiological viability tests and data were fitted to ten inactivation and two regrowth models. The photoactivated complex reduced population, which were unable to regrow. Weibull and Baranyi models were the best to describe the inactivation and regrowth kinetics respectively. In conclusion, data from the kinetic analysis and predictive modelling confirmed that photoactivated chlorophyllin-chitosan complex is a promising non-thermal approach for inactivation of Gram-negative pathogens, since no bacterial regrowth after treatment has been predicted.
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http://dx.doi.org/10.17113/ftb.58.01.20.6374 | DOI Listing |
Food Technol Biotechnol
March 2020
Institute of Computer Science, Vilnius University, Didlaukio g. 47, 08303 Vilnius, Lithuania.
The study focuses on predictive modelling of inactivation of after treatment with chlorophyllin-chitosan complex and visible light. cells were incubated with chlorophyllin-chitosan complex (0.001% chlorophyllin and 0.
View Article and Find Full Text PDFJ Photochem Photobiol B
January 2020
Institute of Photonics and Nanotechnology, Vilnius University, Sauletekio 10, 10223 Vilnius, Lithuania; Faculty of Mathematics and Informatics, Vilnius University, Naugarduko g. 24, Vilnius 03225, Lithuania. Electronic address:
The present study clearly demonstrated the significant antifungal activity of chlorophyllin-chitosan complex (Chl-CHS) after activation with visible light. This phenomenon afterwards was successfully applied for better microbial control of highly popular food- germinated wheat sprouts. Obtained results indicated that photoactivated Chl-CHS complex (0.
View Article and Find Full Text PDFPhotochem Photobiol Sci
April 2016
Vilnius University, Institute of Applied Research, Sauletekio 10, 10223, Vilnius, Lithuania.
This study is focused on the novel approaches to enhance the inactivation of the Gram (-) food pathogen Salmonella enterica and harmful molds in vitro and on the surface of strawberries using the chlorophyllin-chitosan complex. Salmonella enterica (∼1 × 10(7) CFU mL(-1)) was incubated with chlorophyllin 1.5 × 10(-5) M (Chl, food additive), chitosan 0.
View Article and Find Full Text PDFJpn J Cancer Res
August 2001
Gene Research Center, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.
View Article and Find Full Text PDFCancer Lett
September 1999
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
Our studies have shown that hemin and chlorophyllin can directly interact with heterocyclic amines (HAs) and prevent their mutagenic actions. Hemin and chlorophyllin can trap HAs efficiently, probably by forming face-to-face complexes with them. The trapping was most clearly demonstrated by use of solid-supported porphyrins, hemin-agarose and chlorophyllin-chitosan.
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