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induce the production of the hairy root through the transformation of plant genomes. In this article, we executed the transcriptome of through RNA-sequencing. RNA-sequencing of generated a total of 2.6 Gb raw data with a 75 bp paired-end sequence. The raw data has been submitted to the SRA database of NCBI with accession number SRR5641651. Reads were generated 2946 unigenes and all unigenes were annotated in the database. The length of transcripts ranged from 90 to 6369 bp, with a median transcript length of 968. The transcripts were annotated through the number of databases to obtain information about SSRs, SNPs, Gene Ontology, Transcription factors, and pathways analysis .
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http://dx.doi.org/10.1016/j.dib.2020.105910 | DOI Listing |
Plant Sci
September 2025
Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan 250014, China. Electronic address:
Salt stress is one of the main abiotic stresses that affects plant growth and development, as well as crop yield. A large number of studies have reported that the WRKY gene family plays significant roles in the plant responses to salt stress, but the underlying mechanisms remain largely unknown, and research on WRKY proteins in sorghum is also limited. In this study, we identified the sorghum gene SbWRKY51, which encodes a group II WRKY transcription factor.
View Article and Find Full Text PDFTheor Appl Genet
September 2025
Agricultural College, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China.
Saline-alkali soil poses a severe threat to the cultivation and yield of soybean, which is an important oilseed and staple crop. As a key metabolic intermediate, S-adenosyl-L-methionine (SAM) and its associated methyltransferases (SAMMTs) play crucial but poorly understood roles in plant stress responses. This study investigated the expression of SAM-depend methyltransferase (SAMMt) family in soybean.
View Article and Find Full Text PDFPlant Cell Environ
September 2025
College of Life Sciences, Northwest A&F University, Yangling, China.
The neuroactive β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP) was first identified in Lathyrus sativus and present also in several Chinese traditional herbs including Panax notoginseng. It exhibit toxicological effects as the causative agent of neurolathyrism when L. sativus was over-consumed under drought-triggered famines or pharmacological effects including neuroprotection and wound healing.
View Article and Find Full Text PDFActa Pharm Sin B
August 2025
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Genetic transformation is a fundamental tool in molecular biology research of medicinal plants. Tailoring transgenic technologies to each distinct medicinal plant would necessitate a substantial investment of time and effort. Here, we present a simple hairy root transformation method that does not require sterile conditions, utilizing strain K599 and the visible RUBY reporter system.
View Article and Find Full Text PDFPlants (Basel)
August 2025
The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.
In molecular biology studies, suitable vectors are fundamental tools; however, most vectors can only express one target gene, which limits the ability to study multiple genes simultaneously within the same plant tissue. The traditional method for achieving multi-gene co-expression involves co-transferring multiple plasmids into plant tissues, but this approach is often inefficient due to the difficulty of successfully transforming multiple plasmids at once. To overcome this limitation, we have developed a series of vectors, called pMAGs (Multigene Assembly Genetic vectors), capable of simultaneously expressing or silencing two or three different genes in plants.
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