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http://dx.doi.org/10.1111/pbi.13433 | DOI Listing |
J Plant Physiol
December 2024
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China; College of Life Science, University of Chinese Academy of Sciences, Beijing, China; China National Botanical Garden, Beijing, China. Electronic address:
Granule-bound starch synthase I (GBSSI) encoding gene Waxy (Wx), which largely regulates the amylose content of rice grains, is a master module determining rice eating and cooking quality (ECQ). Fine-tuning amylose level of grains is an ideal strategy to improve rice quality. Through fine editing of Wx promoter and 5'UTR by CRISPR/Cas9 system, we created 14 types of novel Wx allelic variations, of which MT7 and MT13 were able to alter Wx expression and amylose content of grains.
View Article and Find Full Text PDFBreed Sci
April 2024
Institute of Crop Science, National Agricultural and Food Research Organization, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
Starch properties are the major determinants of grain quality and food characteristics in rice ( L.). Understanding the interactions between genetic regions responsible for starch properties will lead to the development of rice cultivars with desirable characteristics.
View Article and Find Full Text PDFaBIOTECH
June 2024
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031 China.
Unlabelled: Small mutations in the core promoter region of a gene may result in substantial changes in expression strengths. However, targeting TA-rich sequences of core promoters may pose a challenge for Cas9 variants such as SpCas9 and other G-rich PAM-compatible Cas9s. In this study, we engineered a unique FrCas9 system derived from for plant genome editing.
View Article and Find Full Text PDFPlant Sci
November 2023
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China. Electronic address:
Amylose content (AC) is a significant quality trait in starchy crops, affecting their processing and application by the food and non-food industries. Therefore, fine-tuning AC in these crops has become a focus for breeders. Granule-bound starch synthase (GBSS) is the core enzyme that directly determines the AC levels.
View Article and Find Full Text PDFPlant Biotechnol J
November 2023
Starch accounts for up to 90% of the dry weight of rice endosperm and is a key determinant of grain quality. Although starch biosynthesis enzymes have been comprehensively studied, transcriptional regulation of starch-synthesis enzyme-coding genes (SECGs) is largely unknown. In this study, we explored the role of a NAC transcription factor, OsNAC24, in regulating starch biosynthesis in rice.
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