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http://dx.doi.org/10.1002/smll.202002055 | DOI Listing |
Biosens Bioelectron
September 2022
School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, PR China. Electronic address:
Droplet encapsulation of a single cell or bead is widely used in digital detection, single-cell sequencing, and drug screening. However, the encapsulation of particles is totally random restricted by the Poisson distribution. The theoretical possibility of single-particle encapsulation is usually only approximately 10%.
View Article and Find Full Text PDFSci Rep
April 2022
Department of Electrical Engineering, University of Neyshabur, Neyshabur, Iran.
Precise manipulation of microparticles have fundamental applications in the fields of lab-on-a-chip and biomedical engineering. Here, for the first time, we propose a fully operational microfluidic chip equipped with thin magnetic films composed of straight tracks and bends which precisely transports numerous single-particles in the size range of ~ 2.8-20 µm simultaneously, to certain points, synced with the general external three-axial magnetic field.
View Article and Find Full Text PDFToxicol In Vitro
June 2022
Division of Drug Discovery & Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands. Electronic address:
Estrogen receptor alpha (ERα) is often a primary target of endocrine disrupting chemicals (EDCs) and therefore several biochemical and cell-based assays for the detection of chemicals with estrogenic properties have been developed in the past. However, the current approaches are not suitable for the monitoring of pathway activation dynamics, and they are mostly based on expression constructs that lack physiological promoter regulation. We recently developed MCF7 fluorescent reporter cell lines of 3 different green fluorescent protein (GFP)-tagged ERα target genes: GREB1, PGR and TFF1.
View Article and Find Full Text PDFWaste Manag
March 2022
Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.
This research aimed to valorize almond hulls based on a zero-waste strategy towards sustainable agricultural developments for the recovery and production of valuable compounds. For this purpose, the potential to produce four products, including pectin (AHP), phenolic compounds (AHPC), pullulan (PUL), and single-cell protein (SCP), was examined. The acidic extraction factors were optimized using a Box-Behnken design for the simultaneous extraction of AHP and AHPC, and the obtained results showed that the maximum AHP (26.
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