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Article Abstract
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop CR-mediated inkage of barcoded dapters o nucleic acid lements for uencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260977 | PMC |
| http://dx.doi.org/10.1073/pnas.1918068117 | DOI Listing |
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