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Identifying patterns of fearful behaviors early and accurately is essential to identify children who may be at increased risk for psychopathology. Previous work focused on the total amount of fear by using composites across time. However, considering the temporal dynamics of fear expression might offer novel insights into the identification of children at risk. One hundred and twenty-five toddlers participated in high- and low-fear tasks. Data were modeled using a novel two-step approach. First, a hidden Markov model estimated latent fear states and transitions across states over time. Results revealed children's behavior was best represented by six behavioral states. Next, these states were analyzed using sequence clustering to identify groups of children with similar dynamic trajectories through the states. A four-cluster solution found groups of children varied in fear response and regulation process: "external regulators" (using the caregiver as a regulation tool), "low reactive" (low reaction to stimulus), "fearful explorers" (managing their own internal state with minimal assistance from the caregiver), and "high fear" (fearful/at-caregiver state regardless of task). The combination of analytic tools enabled fine-grained examination of the processes of fearful temperament. These insights may help prevention programs target behaviors that perpetuate anxious behavior in the moment.
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http://dx.doi.org/10.1111/infa.12328 | DOI Listing |
PLoS Pathog
September 2025
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
Macrophages are professional phagocytes that play a major role in engulfing and eliminating invading pathogens. Some intracellular pathogens, such as Salmonella enterica serovar Typhimurium, exploit macrophages as niches for their replication, which requires precise and dynamic modulation of bacterial gene expression in order to resist the hostile intracellular environment. Here, we present a comprehensive analysis of the global transcriptome of S.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Biomedical Data Intelligence, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Capturing the dynamic changes in patients' internal states as they approach death due to fatal diseases remains a major challenge in understanding individual pathologies and improving end-of-life care. However, existing methods primarily focus on specific test values or organ dysfunction markers, failing to provide a comprehensive view of the evolving internal state preceding death. To address this, we analyzed electronic health record (EHR) data from a single institution, including 8,976 cancer patients and 77 laboratory parameters, by constructing continuous mortality prediction models based on gradient-boosting decision trees and leveraging them for temporal analyses.
View Article and Find Full Text PDFPLoS One
September 2025
FAMERP- Faculty of Medicine of São José do Rio Preto, Brazil.
Background: Interprofessional Education (IPE) is widely recognized as essential for fostering collaborative healthcare practices and improving patient outcomes. Despite its acknowledged importance, there remains a notable scarcity of longitudinal research assessing medical students' readiness for IPE across distinct educational stages, particularly within diverse global contexts like Brazil.
Aim: This study sought to address this gap by longitudinally mapping and analyzing the evolution of medical students' readiness for interprofessional learning throughout their academic training at a Brazilian university.
PLoS One
September 2025
Biaoxin Science & Technology (Beijing) Co., Ltd, Beijing, China.
This study examines China's national standard development from 2001 to 2023. Using machine splitting and location assignment technology, the Dagum Gini coefficient and its decomposition methods, and traditional and spatial Markov chain estimation methods, we identify the spatiotemporal disparities and dynamic transition characteristics of the contribution levels to national standard development across China's eight comprehensive economic zones. The findings provide a reference for promoting regional coordinated sustainable development and high-quality economic transformation.
View Article and Find Full Text PDFPLoS Biol
September 2025
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
Morphogenetic information arises from a combination of genetically encoded cellular properties and emergent cellular behaviors. The spatio-temporal implementation of this information is critical to ensure robust, reproducible tissue shapes, yet the principles underlying its organization remain unknown. We investigated this principle using the mouse auditory epithelium, the organ of Corti (OC).
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