Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
In order to increase the successful development of recombinant antibodies and fragments, it seems fundamental to enhance their expression and/or biophysical properties, such as the thermal, chemical, and pH stabilities. In this study, we employed a method bases on replacing the antibody framework region sequences, in order to promote more particularly single-chain Fragment variable (scFv) product quality. We provide evidence that mutations of the VH- C-C' loop might significantly improve the prokaryote production of well-folded and functional fragments with a production yield multiplied by 27 times. Additional mutations are accountable for an increase in the thermal (+19.6 °C) and chemical (+1.9 M) stabilities have also been identified. Furthermore, the hereby-produced fragments have shown to remain stable at a pH of 2.0, which avoids molecule functional and structural impairments during the purification process. Lastly, this study provides relevant information to the understanding of the relationship between the antibodies amino acid sequences and their respective biophysical properties.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344962 | PMC |
| http://dx.doi.org/10.3390/antib9020009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Core-Level Photoelectron Angular Distributions from Bulk-Solvated to Surface-Active Aqueous Potassium Carboxylate Salts.
J Phys Chem B
September 2025
MAX IV Laboratory, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Photoelectron angular distributions are reported for a series of aqueous potassium carboxylate solutions, ranging from bulk-solvated to strongly surface-active species. The quantitative information determined from this work demonstrates how the measured photoelectron angular distributions are influenced by the ions' increasing propensity for the surface in aqueous solutions. Our study provides insight into the relative depth and location of the carboxylate functional group, which is valuable for investigating the adsorption of organic molecules at liquid-vapor interfaces.
View Article and Find Full Text PDFPhosphorothioate-Modified DNA Aptamer-Based PROTACs for Targeted Degradation of Estrogen Receptor α.
Bioconjug Chem
September 2025
Division of Organic Chemistry, National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki 210-9501, Kanagawa, Japan.
Proteolysis-targeting chimeras (PROTACs) have emerged as a powerful modality for selectively degrading intracellular proteins via the ubiquitin-proteasome system. However, their development is often hindered by the limited availability of high-affinity small-molecule ligands, particularly for challenging targets, such as transcription factors. Aptamers─synthetic oligonucleotides with high affinity and specificity─offer a promising alternative as target-binding modules in the PROTAC design.
View Article and Find Full Text PDFDefining breast epithelial cell types in the single-cell era.
Dev Cell
September 2025
Department of Pharmacology, University of Cambridge, Cambridge, UK; Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Electronic address:
Single-cell studies on breast tissue have contributed to a change in our understanding of breast epithelial diversity that has, in turn, precipitated a lack of consensus on breast cell types. The confusion surrounding this issue highlights a possible challenge for advancing breast atlas efforts. In this perspective, we present our consensus on the identities, properties, and naming conventions for breast epithelial cell types and propose goals for future atlas endeavors.
View Article and Find Full Text PDFSynthesis and Optical Properties of Unsymmetric Aromatically π-Extended BODIPY.
J Org Chem
September 2025
School of Chemical and Biopharmaceutical Sciences, Technological University Dublin, City Campus, Grangegorman, Dublin D07 EWV4, Ireland.
A series of unsymmetrically substituted BODIPY dyes featuring fused benzo- or naphtho-fragments on one pyrrolic unit were synthesized from the corresponding pyrrolic precursors. The synthetic route was optimized using a modular approach based on the condensation of formylpyrroles with alkylpyrroles, enabling the identification of precursor combinations that minimize byproduct formation and improve preparative yields. The resulting benzo- and naphtho-fused BODIPYs display intense fluorescence in the red region, with emission maxima spanning 590-680 nm and fluorescence quantum yields ranging from 0.
View Article and Find Full Text PDFMultifunctional Hydrogel Flakes: An Innovative Approach to Localized Delivery of Temozolomide.
ACS Appl Mater Interfaces
September 2025
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, Kraków 30-387, Poland.
The multifunctional systems presented here introduce an innovative and deeply thought-out approach to the more effective and safer use of temozolomide (TMZ) in treating glioma. The developed hydrogel-based flakes were designed to address the issues of local GBL therapy, bacterial neuroinfections, and the bleeding control needed during tumor resection. The materials obtained comprise TMZ and vancomycin (VANC) loaded into cyclodextrin/polymeric capsules and embedded into gelatin/hyaluronic acid/chitosan-based hydrogel films cross-linked with genipin.
View Article and Find Full Text PDF