Preparation of core-shell FeO@poly(dopamine) magnetic nanoparticles for biosensor construction.

Novel core-shell FeO@poly(dopamine) magnetic nanoparticles were prepared through an in situ self-polymerization method. The hybrid nanomaterial showed an average core diameter of 11 ± 3 nm and a polymer thin film thickness of 1.8 ± 0.2 nm. The core-shell nanoparticles were employed as solid supports for the covalent immobilization of horseradish peroxidase (HRP), and the resulting biofunctionalized magnetic nanoparticles were employed to construct an amperometric biosensor for HO. The enzyme biosensor showed a high sensitivity of 442.14 mA M cm, a low limit of detection of 182 nM, a wide linear range from 6.0 × 10 to 8.0 × 10 M and high stability for 1 month.