Oxidative free radicals scavenging activity ( and assay of standardized fractions from the seeds of Argyreia speciosa (Ghav-patta) a traditional Indian medicine.

Traditional Pertinence: Sweet (Linn.), belongs to the family convolvulaceae, a traditional Indian medicinal herb, has been used to treat acute/chronic ulcers, gonorrhea, rheumatoid arthritis and several nervous disorders having a long history.

Aim Of The Study: A broad spectrum approach of this work was to find out the antioxidant activity of seeds, and antioxidant assay were performed.

Material And Methods: Total phenolic content (TPC), reducing power (RP), antioxidant activity (AOA), (superoxide anion), DPPḢ (1,1-diphenyl-2-picrylhydrazyl) and ˙OH (hydroxyl) radicals scavenging activities, GSH (glutathione), CAT (catalase), SOD (superoxide dismutase) and LPO (lipid peroxidase) are the major parameters which were studied for determining and antioxidant property of seed extract & their six fractions obtained from . Carbon tetrachloride (CCl) induced rat model was used to determine antioxidant assay of extract and its fractions.

Results: Butanol fraction (AS-BF) showed strong antioxidant property and protected oxidative DNA damage. AS-BF was found best as compared to all other fraction for determining antioxidant property of seeds with the reduction in lipid peroxide formation and increment in GSH, CAT and SOD. AS-BF showed the presence of phenolic compounds gallic acid, chlorogenic acid, and ellagic acid.

Conclusion: From these results, it was proved that seeds prevent tissue damage due to oxidative stress with strong antioxidant activity.