Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Emiliania huxleyi is a globally important coccolithophore and one of the most successful eukaryotic organisms in the modern oceans. Despite a large body of work on this organism, including the sequencing of its genome, the tools required for forward and reverse functional genetic studies are still undeveloped. Here we present an optimized method for the clonal isolation of E. huxleyi by plating on solid medium. We demonstrate the utility of this method for a variety of strains including haploid, calcifying-diploid, and noncalcifying diploid strains. We show that, in contrast to previous studies, no changes in cell ploidy status occur when the cells are plated. Our method will greatly aid attempts to elucidate the genetic basis of the remarkable physiology of E. huxleyi by forward and reverse genetic approaches.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jpy.12942 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bacteria induce an amoeboid phase in coccolithophores that persists after bloom collapse.
Sci Adv
August 2025
Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich, Switzerland.
Coccolithophores, including bloom-forming species, (formerly ), contribute ~1 to 10% of phytoplankton biomass and are critical for oceanic biogeochemical cycles. is a model system for investigating algal-bacterial-viral interactions and responses to environmental changes and follows a biphasic lifecycle with motile haploid and nonmotile diploid phases. Here, we report a third, "amoeboid" phase: Light and electron microscopy revealed haploid cells rapidly transitioning to an elongated amoeboid cell with reduced motility.
View Article and Find Full Text PDFMapping the transcriptional landscape of algal resistance to viral infection reveals a core expression program.
New Phytol
August 2025
Department of Plant and Environmental Sciences, Weizmann Institute of Science, 7610001, Rehovot, Israel.
Algal blooms and their demise by viruses drive global-scale ecological processes in the ocean. These blooms form the foundation of marine food webs, regulate microbial communities, and shape biogeochemical cycles. Although algal populations are constantly infected by viruses, resistant subpopulations frequently emerge after the infection.
View Article and Find Full Text PDFContext-dependent allelopathy in algal interactions: Insights from laboratory and natural phytoplankton communities.
Harmful Algae
September 2025
Institute for Inorganic and Analytical Chemistry, Bioorganic Analytics, Friedrich Schiller University Jena, Jena 07743, Germany. Electronic address:
A key aspect of algal biology is the production of chemicals used for communication and regulation of interactions. Allelopathic chemicals can facilitate competition between algae, influence community composition and drive succession in the phytoplankton. Much is still unknown about the identities of these allelochemicals, and even the ecological relevance of laboratory derived results is often unclear.
View Article and Find Full Text PDFAgglomerated loricae of the tintinnids and from North Atlantic, tropical Pacific and Southern Ocean waters.
J Plankton Res
August 2025
Aix Marseille Université, Université de Toulon, CNRS, IRD, MIO UM 110, 163 avenue de Luminy, 13288 Marseille, France.
Some 30 species of planktonic tintinnids agglomerate coccoliths and diatom fragments on to their loricae, which have fascinated scientists for centuries. We here provide semi-quantitative scanning electron microscopic observations on tintinnid lorica agglomerations by the small Southern Ocean cf. Hada, intermediate sized North Atlantic (Cleve) Kofoid and Campbell, and larger Southern Ocean Ehrenberg and tropical Pacific Haeckel.
View Article and Find Full Text PDFEvaluating the Antiviral Activity of Termin-8 and Finio Against a Surrogate ASFV-like Algal Virus.
Pathogens
July 2025
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
The objective of this study was to evaluate the time-course of incubation for the potential preventative mitigation of megaviruses using Termin-8 (a formaldehyde-based product) and Finio (non-formaldehyde solution) from Anitox. Emiliania huxleyi virus (EhV), an algal surrogate for African swine fever virus (ASFV), was treated with the recommended concentrations of Termin-8 (0.1% to 0.
View Article and Find Full Text PDF