Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations. Here, we describe a method for using a phage-display platform to assess antibody specificity in human serum. We describe the method with reference to the bacteriophage MS2 virus-like particle (VLP) platform, but it can be adapted to other phage-display technologies as well.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-9853-1_9 | DOI Listing |
Publication Analysis
Top Keywords
antibody specificity
8
specificity human
8
human serum
8
affinity selection
8
phage-display technologies
8
describe method
8
assessing antibody
4
serum deep
4
deep sequence-coupled
4
sequence-coupled biopanning
4