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Modification of the rice genome by Agrobacterium-mediated transformation is a general technique that can be easily performed today. Successful methods were established by vigorous studies on the culture system and the elucidation of Agrobacterium transformation mechanisms. This section provides a detailed description of routine and efficient rice transformation protocols by Agrobacterium. This method uses mature seeds as a material and can be applied to many japonica and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.
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http://dx.doi.org/10.1007/978-1-4939-9865-4_17 | DOI Listing |
Plant Sci
September 2025
Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan 250014, China. Electronic address:
Salt stress is one of the main abiotic stresses that affects plant growth and development, as well as crop yield. A large number of studies have reported that the WRKY gene family plays significant roles in the plant responses to salt stress, but the underlying mechanisms remain largely unknown, and research on WRKY proteins in sorghum is also limited. In this study, we identified the sorghum gene SbWRKY51, which encodes a group II WRKY transcription factor.
View Article and Find Full Text PDFPlant Environ Interact
October 2025
Discipline of Plant Pathology, College of Agriculture, Engineering & Science; School of Agricultural, Earth and Environmental Sciences University of KwaZulu-Natal Pietermaritzburg Republic of South Africa.
Asian soybean rust (ASR) is caused by the biotrophic fungus Syd. & P. Syd.
View Article and Find Full Text PDFActa Pharm Sin B
August 2025
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Genetic transformation is a fundamental tool in molecular biology research of medicinal plants. Tailoring transgenic technologies to each distinct medicinal plant would necessitate a substantial investment of time and effort. Here, we present a simple hairy root transformation method that does not require sterile conditions, utilizing strain K599 and the visible RUBY reporter system.
View Article and Find Full Text PDFProtein Expr Purif
August 2025
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address
Plant-based expression systems offer a promising platform for producing therapeutic glycoproteins with human-compatible glycosylation patterns. This study aimed to engineer tobacco plants (Nicotiana tabacum cv. Yunyan 87) to modify glycosylation pathways for the production of glycoproteins with reduced immunogenicity, enhancing their potential for therapeutic applications.
View Article and Find Full Text PDFPlants (Basel)
August 2025
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico.
Cav., commonly known as "cancer herb" in indigenous communities, has long been used for its medicinal properties. The biotechnological production of its bioactive compounds through genetic transformation represents a valuable approach for obtaining pharmacologically relevant substances.
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