Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
and cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of Pb18, Pb3, and Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in EVs, whereas for there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678485 | PMC |
| http://dx.doi.org/10.3390/cells8070765 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification and Characterization of Five Previously Unrecorded Species of Subgenus Isolated in Korea.
Mycobiology
September 2025
Department of Plant Medicine, Kyungpook National University, Daegu, Korea.
In a survey of freshwater and near-freshwater soils in Chungbuk, Gyeonggi, Gangwon, Gyeongbuk, and Gyeongnam provinces in Korea, seven fungal strains were isolated and identified as members of the genus based on the internal transcribed spacer (ITS) regions sequence analyses. Identification was performed through observing morphological characteristics and conducting phylogenetic analyses based on concatenated partial ITS, β-tubulin, calmodulin, and RNA polymerase II subunit gene sequences. The phylogenetic analyses revealed that strain NNIBRFG6577 was distinct from known species, and based on morphological comparisons with the closest related species, CGMCC 3.
View Article and Find Full Text PDFSTmiR: A Novel XGBoost-based framework for spatially resolved miRNA activity prediction in cancer transcriptomics.
PLoS One
September 2025
Institute of Computational Science and Technology, Guangzhou University, Guangzhou, China.
MicroRNAs (miRNAs) are critical regulators of gene expression in cancer biology, yet their spatial dynamics within tumor microenvironments (TMEs) remain underexplored due to technical limitations in current spatial transcriptomics (ST) technologies. To address this gap, we present STmiR, a novel XGBoost-based framework for spatially resolved miRNA activity prediction. STmiR integrates bulk RNA-seq data (TCGA and CCLE) with spatial transcriptomics profiles to model nonlinear miRNA-mRNA interactions, achieving high predictive accuracy (Spearman's ρ > 0.
View Article and Find Full Text PDFsp. nov., a novel halotolerant, flexirubin-type pigment-producing bacterium of the family .
Int J Syst Evol Microbiol
September 2025
Second Institute of Oceanography, Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources, Hangzhou 310018, PR China.
A Gram-staining-negative, non-motile, aerobic, rod-shaped bacterium, designated 14752, was isolated from a saline lake in Xinjiang Uygur Autonomous Region, China. The strain was subjected to a taxonomic study using a polyphasic approach. Strain 14752 was able to grow at 4-40 ℃ (optimum 28 ℃), pH 6.
View Article and Find Full Text PDFFrom lactation to malignancy: A comparison between healthy and cancerous breast gland at single-cell resolution reveals new issues for tumorigenesis.
FEBS Lett
September 2025
Department of Translational Medicine, University of Ferrara, Italy.
This study, based on datasets from healthy tissues, lactating mammary epithelial cells, and breast cancer phenotypes, investigates mammary gland pathophysiology at single-cell resolution to identify key regulators in breast cancer development and to gain a deeper understanding of its biology and heterogeneity. We suggest that antileukoproteinase (SLPI) has prognostic value associated with metastasis in basal breast cancers. Our analysis highlights the similarity between triple-negative breast cancer cells and mature luminal lactocytes, which share active regulons (SOX2, MTHFD1, POU4F3, and ZNF32), suggesting conserved molecular mechanisms.
View Article and Find Full Text PDFHbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.
Nat Cell Biol
September 2025
Department of Biochemistry, University of Oxford, Oxford, UK.
In mammals, chromosome-wide regulatory mechanisms ensure a balance of X-linked gene dosage between males (XY) and females (XX). In female cells, expression of genes from one of the two X chromosomes is curtailed, with selective accumulation of Xist-RNA, Xist-associated proteins, specific histone modifications (for example, H3K27me3) and Barr body formation observed throughout interphase. Here we show, using chromosome flow-sorting, that during mitosis, Xist-associated proteins dissociate from inactive X (Xi) chromosomes, while high levels of H3K27me3 and increased compaction of the Xi relative to active X (Xa), are retained.
View Article and Find Full Text PDF