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Combining high-resolution single cell tracking experiments with numerical simulations, we show that starvation-induced fruiting body formation in Myxococcus xanthus is a phase separation driven by cells that tune their motility over time. The phase separation can be understood in terms of cell density and a dimensionless Péclet number that captures cell motility through speed and reversal frequency. Our work suggests that M. xanthus takes advantage of a self-driven nonequilibrium phase transition that can be controlled at the single cell level.
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http://dx.doi.org/10.1103/PhysRevLett.122.248102 | DOI Listing |
NAR Genom Bioinform
September 2025
Department of Biotechnology, Indian Institute of Technology Hyderabad, Sangareddy, Telangana, India.
DZ2, a model myxobacterium, has three reported genome assemblies, including two recent complete assemblies (MxDZ2_Tam and MxDZ2_Nan) from the same culture stock. These assemblies misreported their circular nature and differed by 6.4 kb, raising questions about their accuracy.
View Article and Find Full Text PDFAnal Chem
September 2025
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Due to the prevalence and importance of dormant microbial forms in regulating microbial ecosystems, the generation of dormant structures, like spores, has been extensively studied. However, several aspects of the exit of bacterial spores from dormancy, i.e.
View Article and Find Full Text PDFSci Rep
August 2025
Department of Biology, Syracuse University, Syracuse, NY, USA.
Under starvation conditions, a spot of a few million Myxococcus xanthus cells on agar will migrate inward to form aggregates that mature into dome-shaped fruiting bodies. This migration is thought to occur within structures called 'streams,' which are considered crucial for initiating aggregation. The prevailing traffic jam model hypothesizes that intersections of streams cause cell crowding and 'jamming,' thereby initiating the process of aggregate formation.
View Article and Find Full Text PDFbioRxiv
June 2025
Department of Biology, Texas A&M University, College Station, Texas, USA.
Many bacteria form spores to endure unfavorable conditions. While generate endospores through cell division, sporulation in non-Firmicutes remains less understood. The Gram-negative bacterium undergoes sporulation through two distinct mechanisms: rapid sporulation triggered by chemical induction and slow sporulation driven by starvation, both occurring independently of cell division.
View Article and Find Full Text PDFbioRxiv
June 2025
Department of Biology, Texas A&M University, College Station, Texas, USA.
Bacteria generally form only simple multicellular structures lacking the stable cell-cell connections characteristic of eukaryotic tissues. However, when the antibiotic moenomycin modifies peptidoglycan cell wall synthesis, rod-shaped cells of the Gram-negative bacterium become spherical, fuse their outer membranes, and assemble into stable, honeycomb-like lattices resembling eukaryotic tissues. These findings raise the intriguing possibility that some tissue-like organization could have evolved from stress-induced responses in bacterial ancestors.
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