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Objective: To determine the prevalence of resistant pathogens and their antimicrobial susceptibility pattern in an intensive care unit.
Methods: The cross-sectional observational study was conducted at Foundation Hospital, Rawalpindi, Pakistan, from May to September 2016, and comprised tracheal tubes which were collected in sputum culture bottles from patients with clinical findings of ventilator associated pneumonia. The tubes were cultured to locate the resistant pathogens.
Results: A total of 113 different strains of bacteria were isolated from 80 patients. The main isolated bacteria was acinetobacter baumannii 45(39.8%) followed by klebsiella pneumonia 14(12.3%) and methicillin-resistant staphylococcus aureus 13(11.5%). Polymyxin B was the most appropriate drug for treating patients infected with acinetobacter baumannii with a sensitivity of 64% while vancomycin and linez oli dhad 100% sensitivity for methicill in - resistant staphylococcusaureus.
Conclusions: Acinetobacter baumannii was the most prevalent strain in tracheal tubes and polymyxin B was the most effective medicine.
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Vet World
July 2025
Division of Surgery, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Background And Aim: Antibiotic resistance poses a growing threat to wound management in veterinary medicine. Blue light phototherapy has emerged as a non-antibiotic bactericidal alternative with additional benefits for wound healing. However, its effectiveness in clinical veterinary contexts remains inadequately explored.
View Article and Find Full Text PDFPLoS One
September 2025
Los Angeles General Medical Center, Los Angeles, California, United States of America.
Assessing the phagocytosis of microbes by macrophages is an important component of studies of novel immunotherapeutics, antimicrobial drugs, immune effectors, or any immunology related research. Here we define two protocols for measuring in vitro phagocytosis by RAW 246.7 cells - a photographic phagocytosis assay that allows optical measurement of bacterial cells inside of the RAW 246.
View Article and Find Full Text PDFInfect Prev Pract
September 2025
Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P. O. Box 1464, Mwanza, Tanzania.
Background: Hospital surfaces are critical reservoirs of multidrug-resistant pathogens, including third-generation cephalosporin-resistant Gram-negative bacteria (3GC-R-GNB), significantly contributing to healthcare-associated infections (HCAIs). This challenge is pronounced in low- and middle-income countries, where resource constraints limit effective infection prevention and control (IPC) measures. This study screened hospital surfaces for 3GC-R-GNB in selected District Hospitals (DHs) in Mwanza, Tanzania.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Information Technology, Uppsala University, Uppsala, Sweden.
For effective treatment of bacterial infections, it is essential to identify the species causing the infection as early as possible. Current methods typically require hours of overnight culturing of a bacterial sample and a larger quantity of cells to function effectively. This study uses one-hour phase-contrast time-lapses of single-cell bacterial growth collected from microfluidic chip traps, also known as a "mother machine".
View Article and Find Full Text PDFCurr Opin Infect Dis
September 2025
Infectious Diseases Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna.
Purpose Of Review: Sulbactam-durlobactam (SUL-DUR) is a novel β-lactam/β-lactamase inhibitor combination recently approved for carbapenem-resistant Acinetobacter baumannii (CRAB) infections. This review summarizes current knowledge on the optimal use of SUL-DUR, whether administered alone or in combination with carbapenems, particularly imipenem.
Recent Findings: Data from registrational trial demonstrate that SUL-DUR is an effective and well tolerated treatment option for CRAB severe infections.