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Conquering rapid postripeness and deterioration of is quite challenging. We previously observed that methyl jasmonate (MeJA) pretreatment postponed the deterioration of , but the mechanism is unknown. Here, a nontargeted metabolomics analysis by ultrahigh-pressure liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) revealed that MeJA increased the synthesis of malate by inhibiting the decomposition of fumarate and -aconitate. MeJA maintained energy supply by enhancing ATP content and energy charge level and improving hexokinase and glucose-6-phosphate dehydrogenase activities as well. These results promoted ATP supply by maintaining glycolysis, the TCA cycle, and the pentose phosphate pathway. In addition, we revealed that the delayed deterioration was attributed to MeJA treatment which stimulated the energy status of by reducing the respiration rate and nutrient decomposition, thus maintaining energy production. Our results provide a new insight into the role of MeJA treatment in delaying deterioration of through ATP production and supply.
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http://dx.doi.org/10.1021/acs.jafc.9b02872 | DOI Listing |
J Agric Food Chem
September 2025
Laboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, North Carolina 28081, United States.
Barley ( L.) is a nutrient-rich whole grain (WG) with pharmacological potential, partly attributed to its phenolamide content. Using ultrahigh-pressure liquid chromatography coupled with high-resolution electrospray mass spectrometry (UHPLC-HRESI-MS) integrated with Global Natural Products Social (GNPS) molecular networking, we identified 50 phenolamides in WG barley, including 13 agmatines, 14 spermidines, two putrescine conjugates, and 21 hordatines.
View Article and Find Full Text PDFCancer Res Commun
September 2025
AOA Dx, Denver, Colorado.
Unlabelled: Ovarian cancer is the fifth leading cause of cancer-related deaths among women. Most patients are diagnosed at late stage (III/IV), resulting in a 5-year survival rate below 30%. This is driven by the presentation of vague abdominal symptoms that confound diagnosis at early stages (I/II) and a shortage of robust biomarkers.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
September 2025
Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences (SLU), Uppsala SE-75007, Sweden.
High-resolution mass spectrometry (HRMS) is critical for the identification and characterization of (un)known organic chemicals. In this regard, ensuring high mass accuracy in HRMS instruments is essential for reliable results in nontarget and suspect screening. This study presents a practical approach for evaluating and maintaining mass accuracy over time using ultrahigh pressure liquid chromatography coupled with electrospray ionization Orbitrap HRMS.
View Article and Find Full Text PDFJ Agric Food Chem
July 2025
The Australian Wine Research Institute, P.O. Box 46, Glenside, South Australia 5065, Australia.
Thiophenols were recently identified in wine as undesirable compounds contributing to the ashy aftertaste associated with smoke taint. These potential odorants enhanced the unpleasant sensory properties associated with smoke taint at ultratrace (ng/L) levels when present in wine with volatile phenols, but accurate quantification of volatile sulfur compounds at this concentration remains challenging. A simple, stable isotope dilution assay (SIDA) method employing ultrahigh-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated for accurate quantification of six thiophenols (benzenethiol, 2-methylbenzenethiol, 3-methylbenzenethiol, 4-methylbenzenethiol, 2-methoxybenzenethiol, and methylsulfanylbenzene) using N-ethylmaleimide (NEM) as a derivatizing agent and a chemically matched deuterated analogue (-benzenethiol) as the internal standard.
View Article and Find Full Text PDFACS Omega
June 2025
School of Mechanical Engineering, University of Campinas (UNICAMP), Rua Mendeleyev, 200 - CEP 13083-860, Cidade Universitária "Zeferino Vaz", Barão Geraldo, Campinas, SP 13086-002, Brazil.
Several studies have been conducted to identify an efficient method for reducing particulate emissions from vehicle exhaust gases, which are significant contributors to air pollution in large urban areas. One promising approach involves using supercritical combustion, injecting fuel directly at its critical temperature and pressure. Supercritical fluids possess a lower viscosity and surface tension than liquids and higher diffusion rates, facilitating a more uniform mixture distribution, enhancing thermal efficiency, and reducing particulate emissions.
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