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Article Abstract
A series of tetracationic quadrupolar chromophores containing three-coordinate boron π-acceptors linked by different π-bridges, namely 4,4'-biphenyl, 2,7-pyrene, 2,7-fluorene, 3,6-carbazole and 5,5'-di(thien-2-yl)-3,6-diketopyrrolopyrrole, were synthesized. While their neutral precursors displayed highly solvatochromic fluorescence, the water-soluble tetracationic target molecules , did not, but their emission colour could be tuned from blue to pink by changing the π-bridge. Compound , containing the diketopyrrolopyrrole bridge, exhibits the most red-shifted absorption and emission maxima and the largest two-photon absorption cross-section (4560 GM at 740 nm in MeCN). Confocal laser scanning fluorescence microscopy studies in live cells confirm localization of the dye at the lysosome. Moreover, the low cytotoxicity, and high photostability of combined with two-photon excited fluorescence imaging studies demonstrate its excellent potential for lysosomal imaging in live cells.
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| http://dx.doi.org/10.1039/c9sc00793h | DOI Listing |
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