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Monitoring of food quality, in particular, milk quality, is critical in order to maintain food safety and human health. To guarantee quality and safety of milk products and at the same time deliver those as soon as possible, rapid analysis methods as well as sensitive, reliable, cost-effective, easy-to-use devices and systems for process control and milk spoilage detection are needed. In this paper, we review different rapid methods, sensors and commercial systems for milk spoilage and microorganism detection. The main focus lies on chemical sensors and biosensors for detection/monitoring of the well-known indicators associated with bacterial growth and milk spoilage such as changes in pH value, conductivity/impedance, adenosine triphosphate level, concentration of dissolved oxygen and produced CO. These sensors offer several advantages, like high sensitivity, fast response time, minimal sample preparation, miniaturization and ability for real-time monitoring of milk spoilage. In addition, electronic-nose- and electronic-tongue systems for the detection of characteristic volatile and non-volatile compounds related to microbial growth and milk spoilage are described. Finally, wireless sensors and color indicators for intelligent packaging are discussed.
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http://dx.doi.org/10.1016/j.bios.2019.04.040 | DOI Listing |
Electrophoresis
September 2025
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, P. R. China.
In dairy products, Bacillus subtilis (B. subtilis) is considered a harmful spoilage bacterium. Consequently, it is imperative to establish highly sensitive and selective approaches for detecting B.
View Article and Find Full Text PDFInt J Food Microbiol
September 2025
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, Shandong, China. Electronic address:
Raw milk is commonly stored at 4 °C prior to processing, a practice that can facilitate psychrotrophic proliferation, and milk physicochemical alterations and quality deterioration. This study aimed to elucidate the dynamic changes and interrelationships among microbiota, physicochemical parameters, and metabolite profiles in raw bovine and goat milk during refrigerated storage at 4 °C over a 5-day period. The results showed that both bovine and goat milk exhibited significant increases in bacterial counts, titratable acidity, zeta potential, and protein particle size, alongside decreases in pH and lipid particle size, as well as changes in color during refrigerated storage at 4 °C.
View Article and Find Full Text PDFProbiotics Antimicrob Proteins
September 2025
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India.
Ethnic fermented foods represent a significant repository for discovering novel probiotic entities. These fermented foods, entrenched in indigenous practices, have conserved a distinct microbiota through generations. Exploration of these fermented foods could yield microbial consortia capable of transforming human health.
View Article and Find Full Text PDFJ Food Sci Technol
October 2025
Food Quality Control Laboratory, ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131 India.
Biomarkers are important tools in almost every biological field, serving as means of understanding biological conditions, responses or processes. Although, applicability and importance of biomarkers in medicine and food are extensively studied and reported in literature, this review will emphasize on biomarkers associated with food and foods of animal origin like meat, fish, milk and egg. Evaluation of quality, safety and adulteration of food commodities is of the utmost importance with the ever-increasing global demand.
View Article and Find Full Text PDFJ Dairy Sci
September 2025
Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853.
Clostridium tyrobutyricum is a spore-forming bacterium and is considered to be one of the main causative agents of late blowing defect (LBD) of hard and semi-hard cheeses. However, the spoilage potential of C. tyrobutyricum appears to be strain dependent.
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