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Staphylococcal food poisoning is a disease that originates significant health and economic losses and is caused by Staphylococcus aureus strains able to produce enterotoxins. The aim of this work is to go further on the study of the volatile exometabolome of S. aureus using an advanced gas chromatographic technique. Enterotoxic and non-enterotoxic strains were assessed. The volatile exometabolome profile comprised 240 volatiles belonging to ten chemical families. This volatiles were mainly by-products of branched-chain amino acids and methionine degradation, pyruvate metabolism, diacetyl pathway, oxidative stress and carotenoid cleavage. Metabolites released by the first two pathways were produced in higher contents by the enterotoxic strains. This study add further insights to S. aureus volatile exometabolome, and also shows that by applying it, it is possible to distinguish strains of S. aureus by the number of produced enterotoxins, which is especially important from the food safety point of view.
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http://dx.doi.org/10.1016/j.biocel.2019.01.007 | DOI Listing |
Arch Microbiol
August 2025
Department of Biochemistry, Faculty of Science, University of Johannesburg, Auckland Park, 2006, South Africa.
Plant growth-promoting rhizobacteria (PGPR) are bacteria known to enhance plant growth via nitrogen fixation, nutrient solubilization, and phytohormone production. Within the rhizosphere, these bacteria engage in complex intra- and interspecies communication, often mediated by volatile organic compounds (VOCs). VOCs influence microbial behavior, metabolism, and stress responses, yet their specific metabolic impacts remain underexplored.
View Article and Find Full Text PDFTalanta
May 2025
Department of Chemistry, Middle East Technical University, Ankara, 06800, Türkiye. Electronic address:
Solid phase microextraction (SPME), as a sampling/sample preparation technique, offers unique solutions for the most challenging applications, including metabolomics studies of living systems. However, for global metabolomics it is critical to use an SPME sampler facilitating the extraction of both volatiles and nonvolatiles, which at the same time is compatible with thermal and solvent-assisted desorption. As a promising universal coating, recently hydrophilic-lipophilic balanced (HLB) particles immobilized in PTFE have been introduced as a new SPME sampler to provide a wide-range of analyte coverage and compatibility with solvent and thermal desorption.
View Article and Find Full Text PDFChem Biodivers
December 2024
Red de Biodiversidad y Sistemática, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, 91073, Veracruz, México.
Efforts are intensifying to identify bioactive microbial metabolites from biocontrol agents to manage plant pathogens in critical crops. This study examined both volatile organic compounds (VOCs) and non-volatile compounds from Metarhizium carneum and Lecanicillium uredinophilum strains for their antimicrobial effects against various phytopathogens and analyzed their exo-metabolomes. M.
View Article and Find Full Text PDFFoods
February 2023
Institut Universitaire de la Vigne et du Vin Jules Guyot, UMR PAM, Université de Bourgogne, 2 Rue Claude Ladrey, 21000 Dijon, France.
Vitamins are major cofactors to numerous key metabolic pathways in enological yeasts, and both thiamine and biotin, notably, are believed to be essential to yeast fermentation and growth, respectively. In order to further assess and clarify their role in winemaking, and in the resulting wine, alcoholic fermentations of a commercial active dried yeast were conducted in synthetic media containing various concentrations of both vitamins. Growth and fermentation kinetics were monitored and proved the essential character of biotin in yeast growth, and of thiamine in fermentation.
View Article and Find Full Text PDFAnal Bioanal Chem
May 2023
Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal.
Microbial metabolomics allows understanding and to comprehensively analyse metabolites, and their related cellular and metabolic processes, that are produced and released to the extracellular environment under specific conditions. In that regard, the main objective of this research is to understand the impact of culture media changes in the metabolic profile of Pedobacter lusitanus NL19 (NL19) and Pedobacter himalayensis MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC × GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25).
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