O versus NO respiration in a continuous microbial enrichment.

Appl Microbiol Biotechnol

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Published: October 2018


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Article Abstract

Despite its ecological importance, essential aspects of microbial NO reduction-such as the effect of O availability on the NO sink capacity of a community-remain unclear. We studied NO vs. aerobic respiration in a chemostat culture to explore (i) the extent to which simultaneous respiration of NO and O can occur, (ii) the mechanism governing the competition for NO and O, and (iii) how the NO-reducing capacity of a community is affected by dynamic oxic/anoxic shifts such as those that may occur during nitrogen removal in wastewater treatment systems. Despite its prolonged growth and enrichment with NO as the sole electron acceptor, the culture readily switched to aerobic respiration upon exposure to O. When supplied simultaneously, NO reduction to N was only detected when the O concentration was limiting the respiration rate. The biomass yields per electron accepted during growth on NO are in agreement with our current knowledge of electron transport chain biochemistry in model denitrifiers like Paracoccus denitrificans. The culture's affinity constant (K) for O was found to be two orders of magnitude lower than the value for NO, explaining the preferential use of O over NO under most environmentally relevant conditions.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153640PMC
http://dx.doi.org/10.1007/s00253-018-9247-3DOI Listing

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