[Genotyping and polymorphism analysis of cytochrome c oxidase subunit Ⅰ gene of from Lincang City in Yunnan Province].

Objective: To analyze the genetic diversity of based on the mitochondria DNA cytochrome c oxidase subunit Ⅰ (mtDNA COⅠ) gene as a molecular marker in Lincang City of Yunnan Province, so as to provide the scientific data for monitoring in local areas.

Methods: The genotypes and polymorphisms of 38 specimens of collected from Mengding Town of Lincang City were analyzed by sequencing COⅠ gene. The phylogenetic tree and genetic distances were produced based on the haplotypes from GenBank and the present study by using the neighbourjoining method with the software MEGA version 6.06.

Results: Totally 31 sequences were acquired in the present study and they produced 3 unique haplotypes. Haplotype 1 showed a higher frequency compared to the others and it accounted for 83.9 % (26/31). The data showed that the least genetic distances ranged from 0 to 0.052 between and 3 haplotypes, as well as the largest genetic distances ranged from 0.021 to 0.239 between and 3 haplotypes. Otherwise, the analysis of the phylogenetic trees based on COⅠ gene sequences of indicated that all of 3 haplotypes clustered into one big clade with that from Japan (GenBank accession number: AB433769), China (GenBank accession number: KT313034) and USA (GenBank accession number: EU523129), which owned the closet relationship amongst them. Their genetic relationships were distantly related to the GenBank's reference sequences of (GenBank accession number: EF514942), (GenBank accession number: EF515059) and so on.

Conclusions: There is a species in Lincang City of Yunnan Province as well as a high genetic diversity amongst the acquired 3 haplotypes in this study.