98%
921
2 minutes
20
With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929371 | PMC |
http://dx.doi.org/10.1107/S2052252518005389 | DOI Listing |
Front Microbiol
August 2025
State Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, China.
While soil microorganisms underpin terrestrial ecosystem functioning, how their functional potential adapts across environmental gradients remains poorly understood, particularly for ubiquitous taxa. Employing a comprehensive metagenomic approach across China's six major terrestrial ecosystems (41 topsoil samples, 0-20 cm depth), we reveal a counterintuitive pattern: oligotrophic environments (deserts, karst) harbor microbiomes with significantly greater metabolic pathway diversity (KEGG) compared to resource-rich ecosystems. We provide a systematic catalog of key functional genes governing biogeochemical cycles in these soils, identifying: 6 core CAZyme genes essential for soil organic carbon (SOC) decomposition and biosynthesis; 62 nitrogen (N)-cycling genes (KOs) across seven critical enzymatic clusters; 15 sulfur (S)-cycling genes (KOs) within three key enzymatic clusters.
View Article and Find Full Text PDFAnn Bot
September 2025
Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.
Background And Aims: Since the Industrial Revolution, rising atmospheric CO₂, warming, and more frequent droughts have significantly impacted ecosystems. While the response of leaf functional traits to these climate change factors have been widely studied, reproductive traits remain relatively understudied, despite their key role in the diversification and distribution of flowering plants. Here, we investigated how elevated CO₂, warming, drought, and their interactions affect floral, leaf and seed traits in two model grassland species.
View Article and Find Full Text PDFMedicine (Baltimore)
September 2025
Department of Urology, The People's Hospital of Longhua, Shenzhen, China.
Growing evidence have indicated the bidirectional relationships between various inflammatory cytokines and prostate cancer (PCa), but the causality between genetic susceptibility to inflammatory cytokines and PCa was still in initial exploratory phase. This bidirectional Mendelian randomization (MR) research was manipulated to draw causative inferences and the effect of direction between 91 inflammatory cytokines and PCa. Genetic data of PCa were originated from a publicly accessible genome-wide association study with 3269 individuals and 459,664 controls, and inflammatory cytokines summarized by a protein quantitative trait locus study were embodied 14,824 participants.
View Article and Find Full Text PDFNano Lett
September 2025
Department of Physics, The University of Tokyo, Hongo, Tokyo 113-0033, Japan.
On-chip terahertz (THz) spectroscopy has attracted growing attention because of its capability of measuring samples far smaller than the Rayleigh diffraction limit. The technique also allows the investigation of nonlinear responses of materials, which is indispensable for the development of ultrafast devices operating with a THz bandwidth. Here, we report the development of an on-chip THz-pump THz-probe spectroscopy technique that enables the study of ultrafast electrical-pulse-induced nonequilibrium phenomena.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Molecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304.
The biophysical properties of single cells are crucial for understanding cellular function and behavior in biology and medicine. However, precise manipulation of cells in 3-D microfluidic environments remains challenging, particularly for heterogeneous populations. Here, we present "Electro-LEV," a unique platform integrating electromagnetic and magnetic levitation principles for dynamic 3-D control of cell position during separation.
View Article and Find Full Text PDF