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Article Abstract
Gelfoam histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam histoculture at the liquid-air interface. The Gelfoam histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601640 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-7745-1_17 | DOI Listing |
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