Optimization of silver nanoparticles biosynthesis mediated by NRC1731 through application of statistical methods: enhancement and characterization.

3 Biotech

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre (NRC), 33 El Bohouth St., Dokki, Giza, 12622 Egypt.

Published: March 2018


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

The fungal-mediated silver nanoparticles (AgNPs) biosynthesis optimization via the application of central composite design (CCD) response surface to develop an effective ecofriendly and inexpensive green process was the aim of the current study. Nanosilver biosynthesis using the NRC1731 cell-free filtrate (CFF) was studied through involving the most parameters affecting the AgNPs green synthesis and its interactions effects. The statistical optimization models showed that using 59.37% of CFF in reaction containing 1.82 mM silver nitrate for 34 h at pH 7.0 is the optimum value to optimize the AgNPs biosynthesis. The obtained AgNPs were characterized by means of electron microscopy, UV/visible spectrophotometry, energy dispersive X-ray analysis and infrared spectroscopy to elucidate its almost spherical shape with diameter of 3-20 nm. The produced AgNPs exhibited a considerable antimicrobial activity against , in addition to .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814388PMC
http://dx.doi.org/10.1007/s13205-018-1158-6DOI Listing

Publication Analysis

Top Keywords

silver nanoparticles
8
agnps biosynthesis
8
agnps
5
optimization silver
4
biosynthesis
4
nanoparticles biosynthesis
4
biosynthesis mediated
4
mediated nrc1731
4
nrc1731 application
4
application statistical
4

Similar Publications

This study demonstrates the successful fabrication of nanostructured Langmuir-Blodgett (LB) films combining the conjugated copolymer poly(9,9-dioctylfluorene--3,4-ethylenedioxythiophene) (PDOF--PEDOT) with spherical and triangular silver nanoparticles (AgNP). The LB technique allowed precise control over the molecular arrangement and distribution of the nanoparticles at the air-water interface, resulting in compact, reproducible and structurally ordered nanocomposite films. The structural and morphological properties of the interfacial monolayers and LB films were investigated using surface pressure-area isotherms, Brewster angle microscopy, polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS) and quartz crystal microbalance.

View Article and Find Full Text PDF

Objectives: Antibiotic resistance towards penicillin has been attempted to counter by chemically modifying ampicillin through the conjugation with silver nanoparticles (AgNPs). The current study optimizes the conditions for synthesizing and characterizing AgNP-ampicillin to quantify the conjugation extent, evaluate the antibacterial efficacy, and explore the underlying antibacterial mechanisms.

Materials And Methods: AgNPs were synthesized from silver nitrate by chemical reduction method, silica-coated with tetraethyl orthosilicate (TEOS) and amine functionalized by (3-aminopropyl) triethoxysilane (APTES), which was then conjugated with ampicillin via the carbodiimide chemistry.

View Article and Find Full Text PDF

Wounds with extensive tissue damage are highly susceptible for microbial infections delaying the process of wound healing. Currently, biomaterials with therapeutic molecules emerged as key players in wound repairing. This work developed a novel collagen-based hydrogel loaded with allicin and silver nanoparticles.

View Article and Find Full Text PDF

Asthma, a respiratory tract disease, is characterized by inflammation and obstruction of airway. Inflammatory cells play a significant role in allergic asthma, and there is no complete cure for asthma. One of the new approaches in medicines is nanoparticle-base treatment.

View Article and Find Full Text PDF

Biosynthesized Silver Nanoparticles Modulate Inflammation in a Palatine Wound Model.

Clin Exp Dent Res

October 2025

Laboratory of Experimental Physiopathology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina state, Brazil.

Objectives: This study aimed to compare the effects of silver nanoparticles (AgNPs) synthesized with Curcumin (Curcuma longa L.) or Açai (Euterpe oleracea) versus a commercial treatment and photobiomodulation in rat palatal wounds.

Methods: In vitro cell viability tests assessed nanoparticle toxicity.

View Article and Find Full Text PDF