Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD⁺ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746773 | PMC |
| http://dx.doi.org/10.3390/bios7040050 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Towards Precision Nutrition: A Novel Smartphone-Connected Biosensor for Point-of-Care Detection of β-Hydroxybutyrate in Human Blood and Saliva.
Sensors (Basel)
July 2025
Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Precision nutrition is an emerging approach that tailors dietary recommendations based on an individual's unique genetic, metabolic, microbiome, and lifestyle factors. β-hydroxybutyrate (β-HB) is a key ketone body produced during fat metabolism, especially in states of fasting, low-carbohydrate intake, or prolonged exercise. Therefore, monitoring β-HB levels provides valuable insights into an individual's metabolic state, making it an essential biomarker for precision and personalized nutrition.
View Article and Find Full Text PDFExpanding the bioanalytical application of β-hydroxybutyrate binding proteins through characterization of their metabolite interactions and site-directed mutagenesis.
Protein Sci
May 2025
Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, North Carolina, USA.
β-hydroxybutyrate binding proteins (BHBBPs) are a newly identified group of periplasmic solute-binding proteins (SBPs) that interact with β-hydroxybutyrate (BHB), a key physiological metabolite. In this study, we systematically characterized the interaction properties of both previously reported and newly identified BHBBPs, including "NovoS" and "EDC10" from Gram-negative bacteria. Following recombinant production, we assessed the specificity and affinity of these proteins against a library of 23 different metabolites using a label-free derivative of differential scanning fluorimetry (nanoDSF).
View Article and Find Full Text PDFEnhanced biosynthesis of poly(3-hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl-CoA availability.
Microb Biotechnol
November 2024
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.
Article Synopsis
- Malonyl-CoA is crucial for producing valuable compounds like polyketides and biofuels, but its availability is limited due to competition in metabolic pathways.
- Researchers modified a genome-reduced strain of Pseudomonas putida to enhance malonyl-CoA levels by knocking out certain genes involved in sugar metabolism, the TCA cycle, and fatty acid biosynthesis.
- A colorimetric screening method using the RppA gene allowed them to identify strains with increased malonyl-CoA and led to greater polymer production, demonstrating an effective strategy to enhance malonyl-CoA-dependent processes.
Sustainable Nanofibril Interfaces for Strain-Resilient and Multimodal Porous Bioelectronics.
Adv Mater
November 2024
Department of Mechanical & Aerospace Engineering, University of Missouri, Columbia, MO, 65201, USA.
Porous soft bioelectronics have attracted significant attention due to their high breathability, long-term biocompatibility, and other unique features inaccessible in nonporous counterparts. However, fabricating high-quality multimodal bioelectronic components that operate stably under strain on porous substrates, along with integrating microfluidics for sweat management, remains challenging. In this study, cellulose nanofibrils (CNF) are explored, biomass-derived sustainable biomaterials, as nanofibril interfaces with unprecedented interfacial robustness to enable high-quality printing of strain-resilient bioelectronics on porous substrates by reducing surface roughness and creating mechanical heterogeneity.
View Article and Find Full Text PDFSkin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat.
Lab Chip
September 2024
Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA.
Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes.
View Article and Find Full Text PDF