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Aim: The aim of this study was to compare canal transportation and centering ability of Twisted files (TF) and ProTaper (PT) files with conventional stainless steel (SS) K files.
Materials And Methods: Thirty mandibular premolar teeth with root curvature of 25 to 35° were selected. The samples were randomly divided into three groups of 10 each according to the type of files used for instrumentation. Group I was instrumented using TF, group II with PT files according to the manufacturer's recommendations, and group III with samples prepared with pre-curved SS K files using a step-back technique. After preparation, the samples were assessed using spiral computed tomography. Three sections were recorded at coronal, middle, and apical levels. Amount of transportation was assessed comparing with preinstrumentation images. Centering ability was calculated using Gambill's formula. The three groups were statistically compared using Student's t-test.
Results: Twisted files showed the least canal transportation and the best centering ability followed by PT file system. Stainless steel K files showed the highest transportation and least centering ability.
Conclusion: Twisted files showed the least amount of transportation and better centering compared with the other file systems.
Clinical Significance: The study gives information of TF, a recently introduced rotary system (2008) with regard to its efficacy in preparing curved root canals. This study has shown that the centering ratio of rotary nickel titanium was better than that of SS files.
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http://dx.doi.org/10.5005/jp-journals-10024-2066 | DOI Listing |
Mol Biol Rep
September 2025
Teaching Veterinary Clinical Complex, CVAS, KVASU, Thrissur, Kerala, 680651, India.
Background: Ear canker in domestic rabbits is caused by infestations of non-burrowing parasitic mites, Psoroptes spp., but the specific species responsible for these infestations remains unclear. This study reports the clinical signs and performs the molecular characterization and phylogenetic analysis of Psoroptes ovis isolated from the ear canal of a domestic rabbit in South India.
View Article and Find Full Text PDFJ Hazard Mater
September 2025
Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea. Electronic address:
While mercury (Hg) concentration and isotope analyses play pivotal roles in understanding contamination levels and Hg sources, complex hydrodynamics often obscure Hg transport pathways from source to sink. We applied hydrodynamic modeling with Hg stable isotopes to unravel source-specific contamination processes and propose effective management strategies in an estuarine system (Yeongil Bay) impacted by Hg-contaminated riverine input (Hyeongsan River) in Korea. Sediment isotope data revealed contributions of three sources: legacy Hg from the river, regional background Hg, and atmospheric Hg sources.
View Article and Find Full Text PDFAntibiotics (Basel)
August 2025
Water Research Center, Department of Irrigation and Drainage Engineering, Autonomous University of Queretaro, Cerro de las Campanas SN, Col. Las Campanas, Santiago de Querétaro 76010, Mexico.
In recent years, the discharge of antibiotics into rivers and irrigation canals has increased. However, few studies have addressed the impact of these compounds on agricultural fields that use such water to meet crop demands. In this study, the transport of two types of gentamicin (pure gentamicin and gentamicin sulfate) was modeled at concentrations of 150 and 300 μL/L, respectively, in a soil with more than 60 years of agricultural use.
View Article and Find Full Text PDFmedRxiv
August 2025
Department of Pathology and Experimental Therapeutics, Bellvitge University Hospital-IDIBELL, Hospitalet de Llobregat, Barcelona, Spain.
Genome-wide association studies (GWAS) have contributed significantly to unraveling the genetic bases of complex diseases such as Parkinson's disease (PD); yet experimental evidence for causation is often elusive. Here, we hypothesized that non-manifesting carriers of a PD-causing mutation in the gene could express genetic modifiers conferring disease protection. Using a pluripotent stem cell-based model, we showed that dopaminergic neurons derived from these individuals were partially protected from the disease in vitro, and that this protective effect is genetically driven.
View Article and Find Full Text PDFCommun Biol
August 2025
Institut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRS, Marseille, France.
Cerebrospinal Fluid-contacting neurons (CSF-cNs) are GABAergic bipolar neurons found, in contact with the cerebrospinal fluid, along the vertebrate medullo-spinal central canal. They express Polycystin Kidney Disease 2-Like 1 channels (PKD2L1), members of the Transient Receptor Potential superfamily, and were shown to modulate motor activity and therefore suggested to act as a novel sensory system. However, in mice, they remain largely uncharacterized and it is crucial to comprehensively characterize their morphological and electrophysiological properties to determine whether they form a homogenous neuronal population and understand their role in the CNS.
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