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Four samples of showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from but highly dissimilar (~23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15-91.20 and 84.54-90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.
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http://dx.doi.org/10.1007/s13337-016-0329-5 | DOI Listing |
Plant Physiol
May 2025
Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
Replication of plant positive-strand RNA viruses occurs in association with intracellular membranes. To date, no versatile technology has been developed to directly label and visualize an active replicase in live plant cells because, in general, replicase function is not retained when it is fused to a protein for fluorescence imaging. We developed a technique to label and image a plant virus replicase during infection using the transiently expressed human influenza hemagglutinin (HA) frankenbody (FB), an antibody fragment that binds the HA epitope.
View Article and Find Full Text PDFNew Phytol
August 2024
Department of Plant Pathology and Microbiology, Texas A&M University, 496 Olsen Blvd, College Station, TX, 77845, USA.
IRE1, BI-1, and bZIP60 monitor compatible plant-potexvirus interactions though recognition of the viral TGB3 protein. This study was undertaken to elucidate the roles of three IRE1 isoforms, the bZIP60U and bZIP60S, and BI-1 roles in genetic reprogramming of cells during potexvirus infection. Experiments were performed using Arabidopsis thaliana knockout lines and Plantago asiatica mosaic virus infectious clone tagged with the green fluorescent protein gene (PlAMV-GFP).
View Article and Find Full Text PDFInt J Mol Sci
February 2024
Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu 183-8509, Japan.
Systemic acquired resistance (SAR) is a plant defense mechanism that provides protection against a broad spectrum of pathogens in distal tissues. Recent studies have revealed a concerted function of salicylic acid (SA) and -hydroxypipecolic acid (NHP) in the establishment of SAR against bacterial pathogens, but it remains unknown whether NHP is also involved in SAR against viruses. We found that the local application of acibenzolar--methyl (ASM), a synthetic analog of SA, suppressed plantago asiatica mosaic virus (PlAMV) infection in the distal leaves of .
View Article and Find Full Text PDFArch Virol
December 2023
Faculty of Bioresource Science, Akita Prefectural University, Akita, Japan.
We demonstrated the infectivity and host adaptation of a viola isolate of Plantago asiatica mosaic virus (PlAMV-Vi) in an asymptomatic host, Nicotiana benthamiana, through long-term serial passages. Serial passaging of a green fluorescent protein-tagged full-length cDNA clone of PlAMV-Vi (PlAMV-Vi) in N. benthamiana plants resulted in the appearance of a new virus line inducing leaf-crinkle symptoms, the Leaf Crinkle (LC) line.
View Article and Find Full Text PDFJ Virol
June 2023
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Plant viruses depend on a number of host factors for successful infection. Deficiency of critical host factors confers recessively inherited viral resistance in plants. For example, loss of () in Arabidopsis thaliana confers resistance to potexviruses.
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