Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Four samples of showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from but highly dissimilar (~23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15-91.20 and 84.54-90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394712PMC
http://dx.doi.org/10.1007/s13337-016-0329-5DOI Listing

Publication Analysis

Top Keywords

plamv
9
plantago asiatica
8
asiatica mosaic
8
mosaic virus
8
isolate plamv
8
plamv isolate
8
isolate gunwi
8
isolate
5
molecular detection
4
detection characterization
4

Similar Publications

Replication of plant positive-strand RNA viruses occurs in association with intracellular membranes. To date, no versatile technology has been developed to directly label and visualize an active replicase in live plant cells because, in general, replicase function is not retained when it is fused to a protein for fluorescence imaging. We developed a technique to label and image a plant virus replicase during infection using the transiently expressed human influenza hemagglutinin (HA) frankenbody (FB), an antibody fragment that binds the HA epitope.

View Article and Find Full Text PDF

IRE1, BI-1, and bZIP60 monitor compatible plant-potexvirus interactions though recognition of the viral TGB3 protein. This study was undertaken to elucidate the roles of three IRE1 isoforms, the bZIP60U and bZIP60S, and BI-1 roles in genetic reprogramming of cells during potexvirus infection. Experiments were performed using Arabidopsis thaliana knockout lines and Plantago asiatica mosaic virus infectious clone tagged with the green fluorescent protein gene (PlAMV-GFP).

View Article and Find Full Text PDF

Systemic acquired resistance (SAR) is a plant defense mechanism that provides protection against a broad spectrum of pathogens in distal tissues. Recent studies have revealed a concerted function of salicylic acid (SA) and -hydroxypipecolic acid (NHP) in the establishment of SAR against bacterial pathogens, but it remains unknown whether NHP is also involved in SAR against viruses. We found that the local application of acibenzolar--methyl (ASM), a synthetic analog of SA, suppressed plantago asiatica mosaic virus (PlAMV) infection in the distal leaves of .

View Article and Find Full Text PDF

We demonstrated the infectivity and host adaptation of a viola isolate of Plantago asiatica mosaic virus (PlAMV-Vi) in an asymptomatic host, Nicotiana benthamiana, through long-term serial passages. Serial passaging of a green fluorescent protein-tagged full-length cDNA clone of PlAMV-Vi (PlAMV-Vi) in N. benthamiana plants resulted in the appearance of a new virus line inducing leaf-crinkle symptoms, the Leaf Crinkle (LC) line.

View Article and Find Full Text PDF

Plant viruses depend on a number of host factors for successful infection. Deficiency of critical host factors confers recessively inherited viral resistance in plants. For example, loss of () in Arabidopsis thaliana confers resistance to potexviruses.

View Article and Find Full Text PDF