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Article Abstract
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of F-labeled VLPs derived from bacteriophage Qβ by F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425944 | PMC |
| http://dx.doi.org/10.1021/jacs.6b11040 | DOI Listing |
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