Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach.

Anal Bioanal Chem

REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal.

Published: September 2015


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8%) and accuracy (recoveries ≥96.7%). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00216-015-8879-8DOI Listing

Publication Analysis

Top Keywords

peanut allergen
8
detection
4
detection peanut
4
allergen ara
4
ara foodstuffs
4
foodstuffs voltammetric
4
voltammetric biosensing
4
biosensing approach
4
approach voltammetric
4
voltammetric biosensor
4

Similar Publications

Background: Uncertainty exists regarding the health-related quality of life (HRQL) benefits of food allergen oral immunotherapy (OIT). Up-to-date meta-analyses incorporating HRQL data from recent randomised trials are lacking.

Methods: Systematic searches of MEDLINE, Embase, CENTRAL and Google Scholar were conducted for food OIT randomised trials (versus any comparator) that measured HRQL with a validated instrument (27 July 2023).

View Article and Find Full Text PDF

Background: Food allergy (FA) significantly impacts quality of life and public health, but data on prevalence trends in Asia remain limited. This study investigated trends in FA prevalence and related allergic conditions among preschool Chinese children over a 15-year period.

Methodology: Cross-sectional surveys were conducted in 2006, 2013, and 2020, targeting nurseries, preschool, and daycare centers across Hong Kong.

View Article and Find Full Text PDF

Background: The increase of allergies is attributed to a multi-factorial pathogenesis. Multi-gene traits, as well as different environmental factors, are regarded as responsible for shaping the development of atopic entities. The microbiome's composition in particular seems to play a decisive role in allergy prevention and immunological defense.

View Article and Find Full Text PDF

Background: The high burden of peanut allergy underscores the need for treatment options that improve patient health-related quality of life (HRQL). However, the modifying effect of sex assigned at birth on treatment-related outcomes remains poorly understood. We sought to investigate whether sex modifies treatment effect on the change in overall and subdomain HRQL during the PPOIT-003 trial.

View Article and Find Full Text PDF