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A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8%) and accuracy (recoveries ≥96.7%). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
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http://dx.doi.org/10.1007/s00216-015-8879-8 | DOI Listing |
Allergy
September 2025
Allergy Immunology, Murdoch Children's Research Institute, Parkville, Australia.
Background: Uncertainty exists regarding the health-related quality of life (HRQL) benefits of food allergen oral immunotherapy (OIT). Up-to-date meta-analyses incorporating HRQL data from recent randomised trials are lacking.
Methods: Systematic searches of MEDLINE, Embase, CENTRAL and Google Scholar were conducted for food OIT randomised trials (versus any comparator) that measured HRQL with a validated instrument (27 July 2023).
Allergy
September 2025
Global Research Organization, Beckman Coulter Life Sciences, Marseille, France.
Pediatr Allergy Immunol
September 2025
Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China.
Background: Food allergy (FA) significantly impacts quality of life and public health, but data on prevalence trends in Asia remain limited. This study investigated trends in FA prevalence and related allergic conditions among preschool Chinese children over a 15-year period.
Methodology: Cross-sectional surveys were conducted in 2006, 2013, and 2020, targeting nurseries, preschool, and daycare centers across Hong Kong.
Pediatr Allergy Immunol
August 2025
Department of Paediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Background: The increase of allergies is attributed to a multi-factorial pathogenesis. Multi-gene traits, as well as different environmental factors, are regarded as responsible for shaping the development of atopic entities. The microbiome's composition in particular seems to play a decisive role in allergy prevention and immunological defense.
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August 2025
Allergy Immunology, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Background: The high burden of peanut allergy underscores the need for treatment options that improve patient health-related quality of life (HRQL). However, the modifying effect of sex assigned at birth on treatment-related outcomes remains poorly understood. We sought to investigate whether sex modifies treatment effect on the change in overall and subdomain HRQL during the PPOIT-003 trial.
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