Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Vitrification is an excellent tool in the IVF laboratory, enabling options and offering flexibility in assisted reproduction. The technology of cryopreservation has been underway since the early 20th century. The advent of vitrification has advanced the expectations in routine clinical practice in the IVF laboratory presenting impressive results both in post-thaw survival, and in clinical pregnancy rates, as well as significantly enhancing clinical results on preimplantation genetic diagnosis (PGD). Contradicting opinions have been published recently on the limitations and potential that vitrification has in the laboratory, as well as on the optimal approach to employ vitrification in IVF. This review aims to present a comprehensive analysis of the practical aspects of vitrification including concerns and options regarding its use on oocytes and embryos while comparing it with the traditional "slow-freezing" cryopreservation technique.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2478/folmed-2014-0023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Frozen potential: embryo research at the crossroads of ethics, regulation and scientific opportunity.
Development
September 2025
MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
In an era of expanding reproductive possibilities, the human embryo has come to represent both immense potential and profound constraint. Advances in medically assisted reproduction (MAR) have led to the cryopreservation of hundreds of thousands of embryos each year, yet many remain unused and are ultimately discarded. Meanwhile, studies aimed at understanding infertility, early human development and preventing miscarriage continue to face significant barriers, with only a small fraction of embryos ever donated to research.
View Article and Find Full Text PDFChromosomal Abnormalities in Couples Experiencing Recurrent Implantation Failure in West of Iran: A Case-Control Study.
Mol Genet Genomic Med
September 2025
Cytogenetic Laboratories, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
Background: Recurrent Implantation Failure (RIF) is defined as the inability to establish pregnancy despite high-quality embryo transfer after the application of at least three consecutive in vitro fertilization (IVF)/intracytoplasmic sperm injection-embryo transfer procedures. Chromosomal abnormalities are one of the primary reasons for pregnancy failure, miscarriage, and birth defects in both natural conception and IVF pregnancies. This study was to evaluate the incidence of chromosomal abnormalities in peripheral blood samples from 100 couples who experienced RIF.
View Article and Find Full Text PDFOptimizing IVF lab workflows through data-driven insights: associations between lab management, procedural timings, and workload with blastulation rates.
Hum Reprod
September 2025
IVIRMA Global Research Alliance, Genera, Clinica Valle Giulia, Rome, Italy.
Study Question: Do IVF laboratory workflows influence the mean blastulation rate per cohort of inseminated metaphase II oocytes (m-BR)?
Summary Answer: Neither the total number of procedures nor the workload per operator affected m-BR; instead, each additional hour in the interval from ovulation trigger to oocyte denudation (range 36-44 h) was associated with a measurable decline, especially beyond the 40-h threshold.
What Is Known Already: Control of laboratory conditions and standardized protocols are essential for optimizing m-BR in IVF. While advancements in technology and culture systems have improved ART outcomes, the effect of laboratory managerial decisions and procedural timing on embryological outcomes remains unclear.
Management Challenges of a Pregnant Woman With Undiagnosed Nephrotic Syndrome: Experience From Saudi Arabia.
Cureus
August 2025
Department of Obstetrics and Gynecology, Ministry of National Guard Health Affairs, Riyadh, SAU.
This case report discusses the overall care of a female patient with nephrotic syndrome secondary to non-pre-eclampsia-related hypertension in pregnancy, emphasizing the challenges and multidisciplinary treatment needed for desired results. The case presented here involves a 32-year-old pregnant woman with a history of unexplained primary infertility who conceived through in vitro fertilization (IVF). At 26 weeks and three days of gestation, she presented with symptoms suggestive of nephrotic syndrome, including lower limb swelling, facial puffiness, oliguria, and dark-colored urine.
View Article and Find Full Text PDFScreening for non-invasive rsRNA biomarkers to assess embryo quality utilizing ultra-sensitive pandora sequencing combined with machine learning.
J Assist Reprod Genet
September 2025
Center for Reproductive Medicine, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
Purpose: Accurate embryo selection is vital for the success of in vitro fertilization (IVF); however, existing morphological scoring methods are inherently subjective and fail to capture underlying molecular alterations. This study aimed to identify non-invasive molecular markers for embryo quality assessment by analyzing highly modified ribosomal small RNAs (rsRNAs) in embryo culture medium using ultra-sensitive sequencing and machine learning.
Methods: Ultra-sensitive Pandora sequencing was employed to profile rsRNAs in embryo culture medium.