98%
921
2 minutes
20
Bacterial whole-cell biosensors (BWBs) have unique advantages over conventional environmental monitoring techniques on the detection of toxicity and bioavailability of contaminants in water and soils. BWBs can also be rapid, sensitive, semiquantitative, cost-effective, and easy to use. In this study, a standard method is described for the detection of contaminants and toxicity in real water and soil samples using Acinetobacter baylyi ADP1-based biosensors.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-62703-712-9_13 | DOI Listing |
Microb Cell Fact
August 2025
Molecular Plant Biology Unit, Department of Life Technologies, University of Turku, Turku, Finland.
Cyanobacteria are emerging as a promising platform for whole-cell biotransformation, harnessing solar energy to drive biocatalytic reactions through recombinant enzymes. However, optimisation remains challenging due to the complexity of the cyanobacterial metabolism and the regulatory framework in which heterologous enzymes operate. While many enzymes have been deployed for light-driven whole-cell biotransformations, the different experimental conditions used between studies make direct comparison and systematic improvement difficult.
View Article and Find Full Text PDFBiotechnol Appl Biochem
August 2025
Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
The use of whole cells represents a modern approach to enzymatic bioconversion for the production of various compounds, particularly pharmaceuticals. In recent decades, the use of wild strains as whole-cell biocatalysts has faced limitations due to challenges such as the lack of control over enzyme production and activity, as well as inefficiencies in enzyme production. As a result, recombinant cells are often employed.
View Article and Find Full Text PDFVaccines (Basel)
August 2025
School of Public Health and Health Sciences, California State University, Dominguez Hills, Carson, CA 90747, USA.
: The whole cell pertussis vaccine was introduced in the United States in the 1940s and switched to the acellular pertussis vaccine partially in 1992 and completely in 1997. This study examines the relationship between the resurgence of pertussis in the United States and the change in the type of pertussis vaccines. : Pertussis cases from 1922 to 2024 were obtained from the CDC's national notifiable disease surveillance system, and vaccination coverage was obtained from the WHO.
View Article and Find Full Text PDFBiosensors (Basel)
August 2025
Department of Environmental Health Science, Konkuk University, Seoul 05029, Republic of Korea.
TtgR, a transcriptional repressor from , plays a key role in regulating multidrug resistance by controlling the expression of genes in response to various ligands. Despite its broad specificity, TtgR represents a promising candidate for the development of transcription factor (TF)-based biosensors. In this study, we utilized TtgR and its native promoter region (P) as genetic components to construct TF-based biosensors in .
View Article and Find Full Text PDFAppl Environ Microbiol
August 2025
Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
There are no licensed vaccines against , a leading cause of children's diarrhea and travelers' diarrhea. To develop a cross-protective vaccine against heterogeneous species and serotypes, we attempted to apply an epitope- and structure-based vaccinology platform, multiepitope fusion antigen, to construct an optimal polyvalent chimeric immunogen with functional epitopes from the key virulence determinants. With invasion plasmid antigens B and D functional epitopes identified in recent studies, in this study, we focused on intracellular spread protein A (IcsA; also known as virulence gene G, VirG), a multifunctional virulence factor that plays roles in bacterial adherence, invasion, and particularly intracellular and intercellular spread.
View Article and Find Full Text PDF