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Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes. | LitMetric
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Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

Chelsie N Geyer , Nancy D Hanson

J Clin Microbiol

Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.

Published: April 2014

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Citations

20

Article Abstract

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993522PMC
http://dx.doi.org/10.1128/JCM.00214-14DOI Listing

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