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Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization.
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http://dx.doi.org/10.1155/2013/178725 | DOI Listing |
Microbiol Spectr
September 2025
Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
Efficient DNA delivery is essential for genetic manipulation of mycobacteria and for dissecting their physiology, pathogenesis, and drug resistance. Although electroporation enables transformation efficiencies exceeding 10⁵ CFU per µg DNA in and , it remains highly inefficient in many nontuberculous mycobacteria (NTM), including . Here, we discovered that NTM such as exhibit exceptional tolerance to ultra-high electric field strengths and that hypertonic preconditioning partially protects cells from electroporation-induced damage.
View Article and Find Full Text PDFTher Adv Infect Dis
September 2025
University of Utah Hospital, Salt Lake City, UT, USA.
A 79-year-old female diagnosed with myelodysplastic syndrome (MDS) and germline GATA2 mutation, on compassionate cobimetinib, was admitted with subacute cough and dyspnea. Chest imaging demonstrated a new, large, left hilar mass and consolidation with scattered diffuse mediastinal, supraclavicular, and hilar lymphadenopathy. A core biopsy of the right supraclavicular lymph node was performed.
View Article and Find Full Text PDFJ Microbiol Immunol Infect
August 2025
Division of Infectious Diseases, Department of Internal Medicine, Linkou Chang Gung Memorial Hospital, 5 Fuxing St., Guishan Dist., Taoyuan 33305, Taiwan; School of Medicine, College of Medicine, Chang Gung University, 259 Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan. Electronic address
Background: Mycobacterium kansasii complex (MKC) is the second common slowly growing mycobacterium associated with pulmonary diseases, typically presenting as chronic, progressive respiratory symptoms with structural lung damage. This study aimed to identify the prognostic factors, genotypes, antimicrobial susceptibility, and treatment outcomes in patients with MKC pulmonary disease (MKC-PD).
Methods: This retrospective cohort study of patients with MKC-PD from January 2016 to August 2021 was conducted at Linkou Chang Gung Memorial Hospital in Taiwan.
A 64-year-old man, with a history of hepatocellular carcinoma and long-term oral lenvatinib treatment, was hospitalised in our institution twice due to 'cough'. During hospitalisation, the patient underwent multiple bronchoscopies, with lavage fluid sent for pathogen testing, which returned negative results. Finally, was detected via next-generation sequencing (NGS).
View Article and Find Full Text PDFTuberculosis (Edinb)
September 2025
Department of Immunology, ICMR-National Institute for Research in Tuberculosis, Chetpet, Chennai, India. Electronic address:
Non-tuberculous mycobacteria (NTM) are emerging pathogens in human and veterinary medicine, with a globally increasing incidence. In India, sporadic studies have identified an upward trend in NTM infections, but accurate prevalence estimates are lacking due to the absence of nationwide surveillance. Non-tuberculous mycobacteria have been reported in clinically healthy cattle and wildlife globally, complicating tuberculosis (TB) diagnostics and surveillance.
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