Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-induced mutations are heritable, with efficiencies of germline transmission reaching as high as 100%. In addition, we extend the power of the RGN system by showing that these nucleases can be used with single-stranded oligodeoxynucleotides (ssODNs) to create precise intended sequence modifications, including single nucleotide substitutions. Finally, we describe and validate simple strategies that improve the targeting range of RGNs from 1 in every 128 basepairs (bps) of random DNA sequence to 1 in every 8 bps. Together, these advances expand the utility of the CRISPR-Cas system in the zebrafish beyond somatic indel formation to heritable and precise genome modifications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706373 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068708 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Precision plant epigenome editing: what, how, and why.
Trends Plant Sci
September 2025
School of Agriculture and Food Sustainability, The University of Queensland, St Lucia, QLD, Australia, 4072. Electronic address:
Advances in genome engineering have paved the way for targeted epigenome engineering, providing fundamental insights into the role of epigenetic modifications in trait inheritance. Engineered epialleles have already delivered stable, heritable changes in agronomic traits. Despite this capacity, progress in the field has not yet achieved its potential, leaving many avenues of research unexplored.
View Article and Find Full Text PDFGenetic and Cellular Architecture of Breast Cancer Risk in Multi-Ancestry Studies of 159,297 Cases and 212,102 Controls.
medRxiv
August 2025
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Breast cancer genome-wide association studies (GWAS) have identified over 200 independent genome-wide significant susceptibility markers. However, most studies have focused on one or two ancestral groups. We examined breast cancer genetic architecture using GWAS summary statistics from African (AFR), East Asian (EAS), European (EUR) and Hispanic/Latina (H/L) samples, totaling 159,297 cases and 212,102 controls, comprising the largest multi-ancestry study of breast cancer to date.
View Article and Find Full Text PDFOptimizing the breeding strategy in Vanaraja female parent line chicken by monitoring the genetic variability for the major performance traits.
Anim Biosci
September 2025
Poultry Genetics and Breeding Division, ICAR-Directorate of Poultry Research, Rajendranagar, Telangana-500030, India.
Objectives: Vanaraja is a highly successful backyard chicken variety developed by crossing males of Vanaraja male line (PD-1) with females of Vanaraja female line (PD-2). Vanaraja was improved over the generations through selection in parent lines. This study was designed to estimate the genetic parameters and trend for major traits in PD-2 line in order to determine the future breeding strategy in the population.
View Article and Find Full Text PDFGenetic and Environmental Contributions to Child Height in Low- and Middle-Income Countries.
Am J Hum Biol
September 2025
Australian Centre for Precision Health, University of South Australia, Adelaide, South Australia, Australia.
Background: Child height is influenced by genetic and environmental factors. However, it is unclear how these factors vary by geographical region and by study design, particularly in low- and middle-income countries (LMIC), where data is scarce. Understanding these variations will aid the identification of factors that may be hindering growth in specific populations.
View Article and Find Full Text PDFGenome-wide association study reveals candidate genes associated with egg-laying performance in Wuhua yellow chicken.
Poult Sci
August 2025
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Guangdong Innovation Centre for Science and Technology of Wuhua Yellow Chicken, School of Life Sciences, Jiaying University, Meizhou, 514015, China.
Egg production traits are economically critical in poultry farming. However, the genetic mechanisms underlying these traits in indigenous chicken breeds remain largely unknown. In this study, we conducted a genome-wide association study (GWAS) using whole-genome sequencing data from 315 Wuhua yellow chickens, an indigenous breed characterized by low egg production but considerable genetic diversity.
View Article and Find Full Text PDF