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Article Abstract
Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bioorthogonal isonitrile-tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743162 | PMC |
| http://dx.doi.org/10.1002/cbic.201300130 | DOI Listing |
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