Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Deep sequencing analysis of an asymptomatic grapevine revealed a virome containing five RNA viruses and a viroid. Of these, Grapevine leafroll-associated virus 7 (GLRaV-7), an unassigned closterovirus, was by far the most prominently represented sequence in the analysis. Graft-inoculation of the infection to another grape variety confirmed the lack of the leafroll disease symptoms, even though GLRaV-7 could be detected in the inoculated indicator plants. A 16,496 nucleotide-long genomic sequence of this virus was determined from the deep sequencing data. Its genome architecture and the sequences encoding its nine predicted proteins were compared with those of other closteroviruses. The comparison revealed that two other viruses, Little cherry virus-1 and Cordyline virus-1 formed a well supported phylogenetic cluster with GLRaV-7.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898237 | PMC |
| http://dx.doi.org/10.1016/j.virusres.2011.10.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Complete Sequence Analysis of Grapevine Leafroll-Associated Virus 4 and Interactions Between the Encoded Proteins.
Viruses
July 2025
National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology of CAAS, Xingcheng 125100, China.
Grapevine leafroll disease is one of the most devastating diseases in the global viticulture industry. Grapevine leafroll-associated virus 4 is one of the main pathogens causing this disease. In this study, we obtained the complete genome sequences of two Chinese isolates of GLRaV-4 from 'Baisainie' and 'Fantasy Seedless' grapevines through high-throughput sequencing and overlapping RT-PCR combined with RACE technology.
View Article and Find Full Text PDFCommon mycorrhizal network transfers virus infection signals between neighboring grapevines.
Microbiol Res
November 2025
Institute for Adriatic Crops and Karst Reclamation, Department of Plant Sciences, Put Duilova 11, Split 21000, Croatia. Electronic address:
Arbuscular common mycorrhizal networks (CMNs) allow plants to exchange signals, enabling responses to biotic and abiotic stresses in neighboring individuals. However, no studies documenting the transmission of signals from virus-infected plants through CMNs have been published so far. The aim of this study was to investigate whether virus-free grapevine plants, connected via CMNs to grapevines infected with grapevine leafroll-associated virus 3 (GLRaV-3), exhibit physiological or molecular responses to the neighboring virus infection.
View Article and Find Full Text PDFGenome characterization of a newly discovered grapevine leafroll-associated virus S, in the genus ampelovirus by high-throughput sequencing.
J Genet Eng Biotechnol
June 2025
Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address:
Background: Transcriptome data obtained from plant samples often contain significant numbers of reads originating from viral genomes, which are typically co-isolated during the RNA extraction process. This occurs through the simultaneous presence of viral RNA alongside host plant RNA, leading to the inclusion of viral sequences in the transcriptomic data.
Methods And Results: Here, we identify a novel member of the genus Ampelovirus, grapevine leafroll-associated virus S (GLRaV-S) by employing the high-throughput sequencing (HTS) of RNA of grape leaves showing leafroll symptoms using a bioinformatic pipeline for plant virus detection.
Development of a Minireplicon for Grapevine Leafroll-Associated Virus 1 and Genetic Analyses of Sequences in the 5' Non-Translated Region Required for Replication.
Phytopathology
August 2025
Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, WA 99350, U.S.A.
Grapevine leafroll-associated virus 1 (GLRaV-1, genus , family ) has a monopartite RNA genome with the size varying among genetic variants between 18,731 and 18,946 nucleotides (nt) and a 5' non-translated region (5'-NTR), varying in length between 857 and 922 nt. This study was undertaken to examine the role of the 5'-NTR in GLRaV-1 replication. For this purpose, a minireplicon cDNA clone, consisting of the 5'-NTR, the replicase gene module, the green fluorescent protein (GFP) reporter gene, and the 3'-NTR, was constructed.
View Article and Find Full Text PDFIn-depth population genetic study of ssp. from the Black Sea region and its virome.
Front Plant Sci
March 2025
Skryabin Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
The spread of cultivated grapevine from primary centers of origin is inevitably accompanied by the range expansion of its pathogens, including viruses. A limited number of wild L. ssp.
View Article and Find Full Text PDF