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Triple resonance cross-polarization for more sensitive 13C MAS NMR spectroscopy of deuterated proteins. | LitMetric
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Triple resonance cross-polarization for more sensitive 13C MAS NMR spectroscopy of deuterated proteins.

Umit Akbey , Francesca Camponeschi , Barth-Jan van Rossum , Hartmut Oschkinat

Chemphyschem

NMR Supported Structural Biology, Leibniz Institut für Molekulare Pharmakologie, Berlin, Germany.

Published: August 2011

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Article Abstract

Save the last WALTZ for me: the use of simultaneous proton and deuterium cross-polarization for (13)C CPMAS NMR spectroscopy in highly deuterated proteins is discussed. The aim of the new method introduced herein, triple-resonance cross-polarization, is to increase the sensitivity of the carbon-detected methods in such systems.

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http://dx.doi.org/10.1002/cphc.201100084DOI Listing

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